Differential interactions of rabbit plasma alpha-1-antiproteinases S and F with porcine trypsin.

Differential interactions of rabbit plasma alpha-1-antiproteinases S and F with porcine trypsin. Research Abstract Details 

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Differential interactions of rabbit plasma alpha-1-antiproteinases S and F with porcine trypsin. Abstract Text:

A Saito,H Sinohara,

Two major forms of rabbit plasma alpha-1-antiproteinase, S and F, were separated by affinity chromatography on Red Sepharose, and their modes of interaction with porcine trypsin were studied. The S form interacted with trypsin much more slowly than the F form, and the resulting complex partially retained the amidolytic and proteolytic activities towards benzoyl-L-arginine p-nitroanilide and remazol brilliant blue hide powder, respectively. This S form-trypsin complex also prevented the inactivation of bound trypsin by soybean trypsin inhibitor. In marked contrast, an equimolar complex of trypsin and the F form retained neither amidolytic nor proteolytic activity. These results suggest that the F form blocks the active site of trypsin while the S form does not bind directly to the active site, thereby preserving the catalytic potential of trypsin. No similar interaction was observed, however, between the S form and either bovine chymotrypsin or porcine pancreatic elastase. Both the S and F forms inactivated these proteinases in a stoichiometric manner with differing inhibitor/proteinase binding ratios. The S form showed about twofold greater capacity to inhibit elastase than the F form, whereas the reverse was the case for chymotrypsin.

Differential interactions of rabbit plasma alpha-1-antiproteinases S and F with porcine trypsin. Publishing Authors By Initials

A Saito,H Sinohara,

For similar peptides: proteinase inhibitory proteins, secretory: alpha 1-antitrypsin research abstracts see: peptides: proteinase inhibitory proteins, secretory: alpha 1-antitrypsin research

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Differential interactions of rabbit plasma alpha-1-antiproteinases S and F with porcine trypsin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 103

Page Numbers: 247-53

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1988

Differential interactions of rabbit plasma alpha-1-antiproteinases S and F with porcine trypsin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Differential interactions of rabbit plasma alpha-1-antiproteinases S and F with porcine trypsin. Keywords Mesh Terms:

KEYWORDS: alpha 1-Antitrypsin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Differential interactions of rabbit plasma alpha-1-antiproteinases S and F with porcine trypsin. Information

Substance Name: Trypsin

Registry Number: EC 3.4.21.4

Grant and Affiliation Information for Differential interactions of rabbit plasma alpha-1-antiproteinases S and F with porcine trypsin.

AFFILIATION: Department of Biochemistry, Kinki University School of Medicine, Osaka.

Country: JAPAN

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MEDLINETA: J Biochem

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