Differential induction of adult and fetal globin gene expression in the human CML cell subline KU-812F/33.

Differential induction of adult and fetal globin gene expression in the human CML cell subline KU-812F/33. Research Abstract Details 

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Differential induction of adult and fetal globin gene expression in the human CML cell subline KU-812F/33. Abstract Text:

T Endo,Y Ishibashi,S Shiokawa,Y Fukumaki,H Okano,

Various chemicals which are known to have positive effects on differentiation of some erythroid cell lines were tested on a human chronic myelogenous leukemia cell line, KU-812F. Succinic acid, 5-azacytidine, daunomycin, and hemin showed a positive effect. Among them, hemin and 5-azacytidine were the most effective inducers for erythroid differentiation of KU-812F cells. Dimethylsulfoxide, cytosine arabinofuranoside, and sodium n-butyrate showed no effect. In addition, subclone KU-812F/33 derived from the KU-812F cell line showed differential expression of the beta- and gamma-globin genes in the presence of either 2 microM 5-azacytidine or 40 microM hemin. Hemoglobin synthesis in differentiated KU-812F/33 cells was analyzed by isoelectric focusing gel electrophoresis, and S1 mapping analysis of beta- and gamma-globin mRNA was performed. After treatment with 5-azacytidine, the beta-globin gene expression was predominantly enhanced (18.75-fold higher level of beta-globin mRNA). After treatment with hemin, the most notable increase was in the gamma-globin gene expression (1.83-fold higher level of gamma-globin mRNA), while no increment of beta-globin was observed.

Differential induction of adult and fetal globin gene expression in the human CML cell subline KU-812F/33. Publishing Authors By Initials

T Endo,Y Ishibashi,S Shiokawa,Y Fukumaki,H Okano,

For similar cells: cells, cultured: tumor cells, cultured research abstracts see: cells: cells, cultured: tumor cells, cultured research

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Differential induction of adult and fetal globin gene expression in the human CML cell subline KU-812F/33. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 115

Page Numbers: 540-4

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1994

Differential induction of adult and fetal globin gene expression in the human CML cell subline KU-812F/33. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Differential induction of adult and fetal globin gene expression in the human CML cell subline KU-812F/33. Keywords Mesh Terms:

KEYWORDS: Tumor Cells, Cultured

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Differential induction of adult and fetal globin gene expression in the human CML cell subline KU-812F/33. Information

Substance Name: Fetal Hemoglobin

Registry Number: 9034-63-3

Grant and Affiliation Information for Differential induction of adult and fetal globin gene expression in the human CML cell subline KU-812F/33.

AFFILIATION: Department of School-Nurse Training, Kyushu Women's Jr. College, Fukuoka.

Country: JAPAN

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MEDLINETA: J Biochem

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