Differential gene expression during terminal erythroid differentiation.

Differential gene expression during terminal erythroid differentiation. Research Abstract Details 

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Differential gene expression during terminal erythroid differentiation. Abstract Text:

S Koury,S Yarlagadda,K Moskalik-Liermo,N Popli,N Kim,C Apolito,A Peterson,X Zhang,P Zu,J Tamburlin,D Bofinger,S Koury,S Yarlagadda,K Moskalik-Liermo,N Popli,N Kim,C Apolito,A Peterson,X Zhang,P Zu,J Tamburlin,D Bofinger,S Koury,S Yarlagadda,K Moskalik-Liermo,N Popli,N Kim,C Apolito,A Peterson,X Zhang,P Zu,J Tamburlin,D Bofinger,

Terminal erythroid differentiation in mammals is the process whereby nucleated precursor cells accumulate erythroid-specific proteins such as hemoglobin, undergo extensive cellular and nuclear remodeling, and ultimately shed their nuclei to form reticulocytes, which then become mature erythrocytes in the circulation. Little is known about the mechanisms that enable erythroblasts to undergo such a transformation. We hypothesized that genes involved in these mechanisms were likely expressed at restricted times during the differentiation process and used differential display reverse transcriptase polymerase chain reaction as a first step in identifying such genes. We identified three differentially expressed cDNAs that we termed late erythroblast (LEB) 1-3. None of these cDNAs were previously identified as being expressed in erythroblasts and their patterns of expression indicated they are likely to be involved in the differentiation process. LEB-1 cDNA was derived from the gene A330102K04Rik (approved gene symbol Apoll1), and shares homology with members of the apolipoprotein L family in humans. LEB-3 cDNA was derived from the novel gene D930015E06Rik, that has no known function. LEB-2 cDNA was derived from the gene ranBP16 (approved gene symbol Xpo7), a nuclear exportin. D930015E06Rik mRNA is also strongly expressed in the testis and was localized to a region of the seminiferous tubule where secondary spermatocytes and early spermatids are found, suggesting a role for D930015E06Rik in spermatogenesis as well as terminal erythroid differentiation. We have thus identified three genes not previously described as being expressed in erythroblasts that could be relevant in elucidating mechanisms involved in terminal erythroid differentiation.

Differential gene expression during terminal erythroid differentiation. Publishing Authors By Initials

S Koury,S Yarlagadda,K Moskalik-Liermo,N Popli,N Kim,C Apolito,A Peterson,X Zhang,P Zu,J Tamburlin,D Bofinger,S Koury,S Yarlagadda,K Moskalik-Liermo,N Popli,N Kim,C Apolito,A Peterson,X Zhang,P Zu,J Tamburlin,D Bofinger,S Koury,S Yarlagadda,K Moskalik-Liermo,N Popli,N Kim,C Apolito,A Peterson,X Zhang,P Zu,J Tamburlin,D Bofinger,

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Differential gene expression during terminal erythroid differentiation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Genomics

VOLUME: 90

Page Numbers: 574-82

Journal Abbreviation: Genomics

ISSN: 0888-7543

DAY: 31

MONTH: 08

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 8800135

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AFFILIATION: Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, Buffalo, NY 14221, USA.

Country: United States

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MEDLINETA: Genomics

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