Differential changes in Plasmodium falciparum var transcription during adaptation to culture.

Differential changes in Plasmodium falciparum var transcription during adaptation to culture. Research Abstract Details 

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Differential changes in Plasmodium falciparum var transcription during adaptation to culture. Abstract Text:

Jennifer M Peters,Elizabeth V Fowler,Darren R Krause,Qin Cheng,Michelle L Gatton,

Plasmodium falciparum erythrocyte membrane protein 1, which is encoded by the var multigene family, is expressed on the surface of P. falciparum-infected erythrocytes and has been implicated in many of the complications associated with falciparum malaria. Transcriptional switching of var is commonly investigated using in vitro cultured parasites, because parasite material from patients is limited. We investigated the affect of short-term in vitro cultivation on var gene transcription in patient samples. A significant reduction in the overall abundance of var transcripts was observed during the first approximately 10 days of culture. The rate of down-regulation was not constant among all var genes; genes with an upsA, -D, and -E 5' flanking region had a significantly faster rate than genes with an upsB or -C flanking region. These results have significant implications for the investigation of associations between var transcription and clinical manifestations using parasites that have been enriched by in vitro culture.

Differential changes in Plasmodium falciparum var transcription during adaptation to culture. Publishing Authors By Initials

JM Peters,EV Fowler,DR Krause,Q Cheng,ML Gatton,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

PUBMED ID PMID:

MEDLINE DATE:

Differential changes in Plasmodium falciparum var transcription during adaptation to culture. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of infectious diseases

VOLUME: 195

Page Numbers: 748-55

Journal Abbreviation: J. Infect. Dis.

ISSN: 0022-1899

DAY: 22

MONTH: 01

YEAR: 2007

Differential changes in Plasmodium falciparum var transcription during adaptation to culture. Information

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LANGUAGE: eng

NlmUniqueID: 413675

Differential changes in Plasmodium falciparum var transcription during adaptation to culture. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Differential changes in Plasmodium falciparum var transcription during adaptation to culture. Information

Substance Name: Protozoan Proteins

Registry Number: 0

Grant and Affiliation Information for Differential changes in Plasmodium falciparum var transcription during adaptation to culture.

AFFILIATION: Malaria Drug Resistance and Chemotherapy Laboratory, Queensland Institute of Medical Research, Herston, Australia.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI047500-05

ACRONYM: AI

MEDLINETA: J Infect Dis

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