Different involvement of the megakaryocytic lineage by the JAK2 V617F mutation in Polycythemia vera, essential thrombocythemia and chronic idiopathic myelofibrosis.

Different involvement of the megakaryocytic lineage by the JAK2 V617F mutation in Polycythemia vera, essential thrombocythemia and chronic idiopathic myelofibrosis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Different involvement of the megakaryocytic lineage by the JAK2 V617F mutation in Polycythemia vera, essential thrombocythemia and chronic idiopathic myelofibrosis. Abstract Text:

Kais Hussein,Kai Brakensiek,Guntram Buesche,Thomas Buhr,Birgitt Wiese,Hans Kreipe,Oliver Bock,Kais Hussein,Kai Brakensiek,Guntram Buesche,Thomas Buhr,Birgitt Wiese,Hans Kreipe,Oliver Bock,

Atypical megakaryocytes provide the histomorphological hallmark of all Philadelphia-chromosome negative chronic myeloproliferative disorder (Ph(-) CMPD) subtypes and have not been studied so far for the JAK2(V617F) mutation. The mutant gene dosage was determined in isolated megakaryocytes from 68 cases of JAK2(+)/Ph(-) CMPD by a pyrosequencing assay. Megakaryocytes from essential thrombocythemia (ET) showed significantly lower levels of mutated JAK2 alleles compared to patients with chronic idiopathic myelofibrosis (cIMF) with manifest fibrosis and polycythemia vera (PV) but not to prefibrotic cIMF. Solely, ET JAK2V617F in megakaryocytes is associated with a PV-like phenotype, and at least in one patient, the JAK2 mutation was exclusively acquired within the megakaryocytic lineage. The overt differences between prefibrotic and fibrotic cIMF suggested a causative role of the gene dosage of mutant JAK2 in fibrotic progression. Megakaryocyte analysis of a follow-up of eight individual cases with sequential biopsies, however, showed that progression to homozygosity of V617F mutated JAK2 and onset of manifest fibrosis appeared to be independent events. We conclude that megakaryocytes might be the predominant or even the exclusive lineage that acquires the JAK2(V617F) mutation in ET and that the JAK2(V617F) evolution to higher gene dosages represents a dynamic and complex process substantially involving megakaryocytes.

Different involvement of the megakaryocytic lineage by the JAK2 V617F mutation in Polycythemia vera, essential thrombocythemia and chronic idiopathic myelofibrosis. Publishing Authors By Initials

K Hussein,K Brakensiek,G Buesche,T Buhr,B Wiese,H Kreipe,O Bock,K Hussein,K Brakensiek,G Buesche,T Buhr,B Wiese,H Kreipe,O Bock,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Different involvement of the megakaryocytic lineage by the JAK2 V617F mutation in Polycythemia vera, essential thrombocythemia and chronic idiopathic myelofibrosis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Annals of hematology

VOLUME: 86

Page Numbers: 245-53

Journal Abbreviation: Ann. Hematol.

ISSN: 1432-0584

DAY: 30

MONTH: 01

YEAR: 2007

Different involvement of the megakaryocytic lineage by the JAK2 V617F mutation in Polycythemia vera, essential thrombocythemia and chronic idiopathic myelofibrosis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9107334

Different involvement of the megakaryocytic lineage by the JAK2 V617F mutation in Polycythemia vera, essential thrombocythemia and chronic idiopathic myelofibrosis. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Different involvement of the megakaryocytic lineage by the JAK2 V617F mutation in Polycythemia vera, essential thrombocythemia and chronic idiopathic myelofibrosis. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Different involvement of the megakaryocytic lineage by the JAK2 V617F mutation in Polycythemia vera, essential thrombocythemia and chronic idiopathic myelofibrosis.

AFFILIATION: Institute of Pathology, Hannover Medical School, Hannover, Germany.

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Ann Hematol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Different involvement of the megakaryocytic lineage by the JAK2 V617F mutation in Polycythemia vera, essential thrombocythemia and chronic idiopathic myelofibrosis Related Publications

• A computational investigation of the geometrical structure and protodeboronation of boroglycine, H2N-CH2-B(OH)2.
• Different involvement of the megakaryocytic lineage by the JAK2 V617F mutation in Polycythemia vera, essential thrombocythemia and chronic idiopathic myelofibrosis.
• Disruption of a tight cluster surrounding tyrosine 131 in the native conformation of antithrombin III activates it for factor Xa inhibition.
• Effectiveness of Various Exposure Metrics in Defining Dose-Response Relationships for Mink (Mustela vison) Exposed to Polychlorinated Biphenyls.
• Effects of P2, a peptide derived from a homophilic binding site in the neural cell adhesion molecule on learning and memory in rats.
• Evaluation of beta-cell mass and function in the Göttingen minipig.
• Expression of allosteric linkage between the sodium ion binding site and exosite I of thrombin during prothrombin activation.
• Faithful transcription initiation from a mitochondrial promoter in transgenic plastids.
• Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway.
• Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons.
• Medical condition is related to treatment preference in cancer patients: implications for quality assessment.
• Neural cell adhesion molecule-180-mediated homophilic binding induces epidermal growth factor receptor (EGFR) down-regulation and uncouples the inhibitory function of EGFR in neurite outgrowth.
• OrganellarGenomeDRAW (OGDRAW): a tool for the easy generation of high-quality custom graphical maps of plastid and mitochondrial genomes.
• Primary vaccination of infants against hepatitis B can be completed using a combined hexavalent diphtheria-tetanus-acellular pertussis-hepatitis B-inactivated poliomyelitis-Haemophilus influenzae type B vaccine.
• Prototypicality in sentence production.
• Reliability of the beighton hypermobility index to determinate the general joint laxity performed by dentists.
• Resting energy expenditure in patients with isolated head injuries and spontaneous intracranial haemorrhages.
• Safety, Reactogenicity and Immunogenicity of the Live Attenuated Combined Measles, Mumps and Rubella Vaccine Containing the RIT 4385 Mumps Strain in Healthy Singaporean Children.
• Serum protein concentration and relative albumin percentage in normal individuals.
• Step into Motion: a randomized trial examining the relative efficacy of Internet vs. print-based physical activity interventions.
• Structure of the boronic acid dimer and the relative stabilities of its conformers.
• Transfer of plastid RNA-editing activity to novel sites suggests a critical role for spacing in editing-site recognition.
• Treatment of Friedländer's pneumonia with streptomycin; report of a case.
• Yeast beta-glucosidase: comparison of the physical-chemical properties of purified constitutive and inducible enzyme.
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• alpha-Hydroxy amides as a novel class of bradykinin B(1) selective antagonists.