Differences in protein mobility between pioneer versus follower growth cones.

Differences in protein mobility between pioneer versus follower growth cones. Research Abstract Details 

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Differences in protein mobility between pioneer versus follower growth cones. Abstract Text:

Rajan P Kulkarni,Magdalena Bak-Maier,Scott E Fraser,

Navigating growth cones need to integrate, process and respond to guidance signals, requiring dynamic information transfer within and between different compartments. Studies have shown that, faced with different navigation challenges, growth cones display dynamic changes in growth kinetics and morphologies. However, it remains unknown whether these are paralleled by differences in their internal molecular dynamics. To examine whether there are protein mobility differences during guidance, we developed multiphoton fluorescence recovery after photobleaching methods to determine molecular diffusion rates in pathfinding growth cones in vivo. Actively navigating growth cones (leaders) have consistently longer recovery times than growth cones that are fasciculated and less actively navigating (followers). Pharmacological perturbations of the cytoskeleton point to actin as the primary modulator of diffusion in differently behaving growth cones. This approach provides a powerful means to quantify mobility of specific proteins in neurons in vivo and reveals that diffusion is important during axon navigation.

Differences in protein mobility between pioneer versus follower growth cones. Publishing Authors By Initials

RP Kulkarni,M Bak-Maier,SE Fraser,

For similar animals: chordata: vertebrates: fishes: cypriniformes: cyprinidae: zebrafish research abstracts see: animals: chordata: vertebrates: fishes: cypriniformes: cyprinidae: zebrafish research

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Differences in protein mobility between pioneer versus follower growth cones. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 1207-12

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 17

MONTH: 01

YEAR: 2007

Differences in protein mobility between pioneer versus follower growth cones. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Differences in protein mobility between pioneer versus follower growth cones. Keywords Mesh Terms:

KEYWORDS: Zebrafish

MESH TERMS: embryology

Chemical & Substance for Abstract: Differences in protein mobility between pioneer versus follower growth cones. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for Differences in protein mobility between pioneer versus follower growth cones.

AFFILIATION: Option in Biochemistry and Molecular Biophysics, Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

Country: United States

AGENCY: United States NICHD

GRANT: HD043897

ACRONYM: HD

MEDLINETA: Proc Natl Acad Sci U S A

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