Diatom-derived carbohydrates as factors affecting bacterial community composition in estuarine sediments.

Diatom-derived carbohydrates as factors affecting bacterial community composition in estuarine sediments. Research Abstract Details 

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Diatom-derived carbohydrates as factors affecting bacterial community composition in estuarine sediments. Abstract Text:

Kelly Haynes,Tanja A Hofmann,Cindy J Smith,Andrew S Ball,Graham J C Underwood,A Mark Osborn,Kelly Haynes,Tanja A Hofmann,Cindy J Smith,Andrew S Ball,Graham J C Underwood,A Mark Osborn,

Microphytobenthic biofilms in estuaries, dominated by epipelic diatoms, are sites of high primary productivity. These diatoms exude large quantities of extracellular polymeric substances (EPS) comprising polysaccharides and glycoproteins, providing a substantial pool of organic carbon available to heterotrophs within the sediment. In this study, sediment slurry microcosms were enriched with either colloidal carbohydrates or colloidal EPS (cEPS) or left unamended. Over 10 days, the fate of these carbohydrates and changes in beta-glucosidase activity were monitored. Terminal restriction fragment length polymorphism (T-RFLP), DNA sequencing, and quantitative PCR (Q-PCR) analysis of 16S rRNA sequences were used to determine whether sediment bacterial communities exhibited compositional shifts in response to the different available carbon sources. Initial heterotrophic activity led to reductions in carbohydrate concentrations in all three microcosms from day 0 to day 2, with some increases in beta-glucosidase activity. During this period, treatment-specific shifts in bacterial community composition were not observed. However, by days 4 and 10, the bacterial community in the cEPS-enriched sediment diverged from those in colloid-enriched and unamended sediments, with Q-PCR analysis showing elevated bacterial numbers in the cEPS-enriched sediment at day 4. Community shifts were attributed to changes in cEPS concentrations and increased beta-glucosidase activity. T-RFLP and sequencing analyses suggested that this shift was not due to a total community response but rather to large increases in the relative abundance of members of the gamma-proteobacteria, particularly Acinetobacter-related bacteria. These experiments suggest that taxon- and substrate-specific responses within the bacterial community are involved in the degradation of diatom-derived extracellular carbohydrates.

Diatom-derived carbohydrates as factors affecting bacterial community composition in estuarine sediments. Publishing Authors By Initials

K Haynes,TA Hofmann,CJ Smith,AS Ball,GJ Underwood,AM Osborn,K Haynes,TA Hofmann,CJ Smith,AS Ball,GJ Underwood,AM Osborn,

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Diatom-derived carbohydrates as factors affecting bacterial community composition in estuarine sediments. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Applied and environmental microbiology

VOLUME: 73

Page Numbers: 6112-24

Journal Abbreviation: Appl. Environ. Microbiol.

ISSN: 0099-2240

DAY: 3

MONTH: 08

YEAR: 2007

Diatom-derived carbohydrates as factors affecting bacterial community composition in estuarine sediments. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Diatom-derived carbohydrates as factors affecting bacterial community composition in estuarine sediments.

AFFILIATION: Department of Animal and Plant Sciences, The University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom.

Country: United States

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MEDLINETA: Appl Environ Microbiol

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