Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2.

Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2. Abstract Text:

Dong Xiao,Mengfeng Li,Anna Herman-Antosiewicz,Jedrzej Antosiewicz,Hui Xiao,Karen L Lew,Yan Zeng,Stanley W Marynowski,Shivendra V Singh,

We have shown recently that diallyl trisulfide (DATS), a cancer-chemopreventive constituent of garlic, inactivates Akt to trigger mitochondrial translocation of proapoptotic protein BAD in human prostate cancer cells. Because Akt activation is implicated in the promotion of endothelial cell survival and angiogenesis, we hypothesized that DATS may inhibit angiogenesis. In the present study, we tested this hypothesis using human umbilical vein endothelial cells (HUVECs) as a model. Survival of HUVECs was reduced significantly in the presence of DATS in a concentration-dependent manner, with an IC50 of approximately 4 microM. The DATS-mediated suppression of HUVEC survival was associated with apoptosis induction characterized by accumulation of subdiploid cells, cytoplasmic histone-associated DNA fragmentation, and cleavage of caspase-3 and poly-(ADP-ribose)-polymerase. The DATS-induced DNA fragmentation was significantly attenuated in the presence of pan-caspase inhibitor zVAD-fmk and specific inhibitors of caspase-9 (zLEHD-fmk) and caspase-8 (zIETD-fmk). DATS treatment inhibited the formation of capillary-like tube structure and migration by HUVECs in association with suppression of vascular endothelial growth factor (VEGF) secretion and VEGF receptor-2 protein level and inactivation of Akt kinase. DATS treatment also caused activation of extracellular signal-regulated kinase 1/2 (ERK1/2) but not c-Jun NH2-terminal kinase (JNK) or p38 mitogen-activated protein kinase (p38MAPK).DATS-mediatedapoptosis induction and inhibition of HUVEC tube formation was partially but statistically significantly attenuated by pharmacologic inhibition of ERK1/2 but not JNK or p38MAPK. The present study demonstrates, for the first time, that DATS has the ability to inhibit angiogenic features of human endothelial cells.

Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2. Publishing Authors By Initials

D Xiao,M Li,A Herman-Antosiewicz,J Antosiewicz,H Xiao,KL Lew,Y Zeng,SW Marynowski,SV Singh,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nutrition and cancer

VOLUME: 55

Page Numbers: 94-107

Journal Abbreviation:

ISSN: 0163-5581

DAY: 3

MONTH: 12

YEAR: 2006

Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7905040

Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2.

AFFILIATION: University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Nutr Cancer

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2 Related Publications

• A definition of the breeding season of Pool Dorset ewes.
• A shift from significance test to hypothesis test through power analysis in medical research.
• Bio-histopathological studies of endometrium in repeat breeding buffaloes (Bubalus bubalis).
• Challenging and Changing Gender Attitudes among Young Men in Mumbai, India.
• Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2.
• ERK/MAPK pathway regulates GABAA receptors.
• Effect of adenotonsillitis on peak exploratory flow rate in children.
• Effects of bull and heparin and sperm concentrations on in vitro fertilization of buffalo (Bubalus bubalis ) oocytes matured in vitro.
• Efficacy of the Hamilton Thorn Motility Analyzer (HTM-2030) for the evaluation of bovine semen.
• Embryo transfer as a means of controlling the transmission of viral infections. XIII. Failure to transmit foot-and-mouth disease virus through the transfer of embryos from viremic donors.
• Expression of the cytoplasmic domain of beta1 integrin induces apoptosis in adult rat ventricular myocytes (ARVM) via the involvement of caspase-8 and mitochondrial death pathway.
• GSNO attenuates EAE disease by S-nitrosylation-mediated modulation of endothelial-monocyte interactions.
• How much canola or cottonseed meals can be used for commercial chicken meat production.
• In patients with mild hypertension, does exercise and a gradual rather than abrupt increase in fatty acid and salt intake cause less rise in cardiovascular risk factors?
• In vitro maturation and fertilization of buffalo oocytes (Bubalus bubalis ): Effects of media, hormones and sera.
• Integrated preconcentration SDS-PAGE of proteins in microchips using photopatterned cross-linked polyacrylamide gels.
• Interaction of maternal protein and carbon monoxide on pup mortality in mice: implications for global infant mortality.
• Mitochondria as targets for detection and treatment of cancer.
• On-chip native gel electrophoresis-based immunoassays for tetanus antibody and toxin.
• Phenethyl isothiocyanate-induced apoptosis in PC-3 human prostate cancer cells is mediated by reactive oxygen species-dependent disruption of the mitochondrial membrane potential.
• Plasma alpha-tocopherol profiles in sheep after oral administration of dl-alpha-tocopheryl acetate and d-alpha-tocopheryl succinate.
• Prophylactic gabapentin for prevention of postoperative nausea and vomiting in patients undergoing laparoscopic cholecystectomy: A randomized, double-blind, placebo-controlled study.
• Proteomic analyses of murine macrophages treated with Bacillus anthracis lethal toxin.
• Proteomic analysis of mitochondria-to-nucleus retrograde response in human cancer.
• Responses of different doses of prostaglandin F(2) alpha on estrus induction, fertility and progesterone levels in subestrous buffaloes.
• Seasonal variation in freezability of buffalo semen.
• Steroid receptor coactivator AIB1 in endometrial carcinoma, hyperplasia and normal endometrium: Correlation with clinicopathologic parameters and biomarkers.
• The mental health challenge in Sri Lanka from working within the disaster area.
• Up-regulation of transient receptor potential canonical 1 (TRPC1) following sarco(endo)plasmic reticulum Ca2+ ATPase 2 gene silencing promotes cell survival: a potential role for TRPC1 in Darier's disease.
• Variation in angiotensin-converting enzyme activity in the testes of prepubertal, pubertal and adult cockerels.