Diallyl disulphide depletes glutathione in Candida albicans: oxidative stress-mediated cell death studied by two-photon microscopy.

Diallyl disulphide depletes glutathione in Candida albicans: oxidative stress-mediated cell death studied by two-photon microscopy. Research Abstract Details 

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Diallyl disulphide depletes glutathione in Candida albicans: oxidative stress-mediated cell death studied by two-photon microscopy. Abstract Text:

Katey M Lemar,Miguel A Aon,Sonia Cortassa,Brian O'Rourke,Carsten T ,David Lloyd,

Using two-photon scanning laser microscopy, we investigated the effect of an Allium sativum (garlic) constituent, diallyl disulphide (DADS), on key physiological functions of the opportunistic pathogen Candida albicans. A short 30 min exposure to 0.5 mM DADS followed by removal induced 70% cell death (50% necrotic, 20% apoptotic) within 2 h, increasing to 75% after 4 h. The early intracellular events associated with DADS-induced cell death were monitored with two-photon fluorescence microscopy to track mitochondrial membrane potential (Deltapsi(m)), reactive oxygen species (ROS) and NADH or reduced glutathione (GSH) under aerobic conditions. DADS treatment decreased intracellular GSH and elevated intracellular ROS levels. Additionally, DADS induced a marked decrease of Deltapsi(m) and lowered respiration in cell suspensions and isolated mitochondria. In vitro kinetic experiments in cell-free extracts suggest that glutathione-S-transferase (GST) is one of the intracellular targets of DADS. Additional targets were also identified, including inhibition of a site or sites between complexes II-IV in the electron transport chain, as well as the mitochondrial ATP-synthase. The results indicate that DADS is an effective antifungal agent able to trigger cell death in Candida, most probably by eliciting oxidative stress as a consequence of thiol depletion and impaired mitochondrial function.

Diallyl disulphide depletes glutathione in Candida albicans: oxidative stress-mediated cell death studied by two-photon microscopy. Publishing Authors By Initials

KM Lemar,MA Aon,S Cortassa,B O'Rourke,CT ,D Lloyd,

For similar biochemical phenomena, metabolism, and nutrition: metabolism: oxidative stress research abstracts see: biochemical phenomena, metabolism, and nutrition: metabolism: oxidative stress research

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Diallyl disulphide depletes glutathione in Candida albicans: oxidative stress-mediated cell death studied by two-photon microscopy. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Yeast (Chichester, England)

VOLUME: 24

Page Numbers: 695-706

Journal Abbreviation: Yeast

ISSN: 0749-503X

DAY: 3

MONTH: Aug

YEAR: 2007

Diallyl disulphide depletes glutathione in Candida albicans: oxidative stress-mediated cell death studied by two-photon microscopy. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8607637

Diallyl disulphide depletes glutathione in Candida albicans: oxidative stress-mediated cell death studied by two-photon microscopy. Keywords Mesh Terms:

KEYWORDS: Oxidative Stress

MESH TERMS: drug effects

Chemical & Substance for Abstract: Diallyl disulphide depletes glutathione in Candida albicans: oxidative stress-mediated cell death studied by two-photon microscopy. Information

Substance Name: Glutathione

Registry Number: 70-18-8

Grant and Affiliation Information for Diallyl disulphide depletes glutathione in Candida albicans: oxidative stress-mediated cell death studied by two-photon microscopy.

AFFILIATION: Microbiology (BIOSI 1), Main Building, Cardiff University, PO Box 915, Cardiff CF10 3TL, UK.

Country: England

AGENCY: United States NHLBI

GRANT: R01-HL54598

ACRONYM: HL

MEDLINETA: Yeast

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