Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories.

Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories. Research Abstract Details 

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Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories. Abstract Text:

Marcus Panning,Thomas Laue,Stephan Olschlager,Markus Eickmann,Stephan Becker,Sabine Raith,Marie-Claude Georges Courbot,Mikael Nilsson,Robin Gopal,Ake Lundkvist,Antonino di Caro,David Brown,Hermann Meyer,Graham Lloyd,Beate M Kummerer,Stephan Gunther,Christian Drosten,Marcus Panning,Thomas Laue,Stephan Olschlager,Markus Eickmann,Stephan Becker,Sabine Raith,Marie-Claude Georges Courbot,Mikael Nilsson,Robin Gopal,Ake Lundkvist,Antonino di Caro,David Brown,Hermann Meyer,Graham Lloyd,Beate M Kummerer,Stephan Gunther,Christian Drosten,Marcus Panning,Thomas Laue,Stephan Olschlager,Markus Eickmann,Stephan Becker,Sabine Raith,Marie-Claude Georges Courbot,Mikael Nilsson,Robin Gopal,Ake Lundkvist,Antonino di Caro,David Brown,Hermann Meyer,Graham Lloyd,Beate M Kummerer,Stephan Gunther,Christian Drosten,

A network of European biosafety level 4 laboratories has designed the first industry-standard molecular assay for all filoviruses species, based on the strain collections of all participants. It uses 5 optimized L gene primers and 3 probes, as well as an internal control with a separate detection probe. Detection limits (probit analysis, 95% detection chance) were as follows: Zaire ebolavirus, 487 copies/mL of plasma; Sudan ebolavirus Maleo, 586 copies/mL; Sudan ebolavirus Gulu, 1128 copies/mL; Cote d'Ivoire ebolavirus, 537 copies/mL; Reston ebolavirus, 4546 copies/mL; Lake Victoria marburgvirus Musoke, 860 copies/mL; and Lake Victoria marburgvirus Ravn, 1551 copies/mL. The assay facilitates reliable detection or exclusion screening of filovirus infections.

Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories. Publishing Authors By Initials

M Panning,T Laue,S Olschlager,M Eickmann,S Becker,S Raith,MC Courbot,M Nilsson,R Gopal,A Lundkvist,A Caro,D Brown,H Meyer,G Lloyd,BM Kummerer,S Gunther,C Drosten,M Panning,T Laue,S Olschlager,M Eickmann,S Becker,S Raith,MC Courbot,M Nilsson,R Gopal,A Lundkvist,A Caro,D Brown,H Meyer,G Lloyd,BM Kummerer,S Gunther,C Drosten,M Panning,T Laue,S Olschlager,M Eickmann,S Becker,S Raith,MC Courbot,M Nilsson,R Gopal,A Lundkvist,A Caro,D Brown,H Meyer,G Lloyd,BM Kummerer,S Gunther,C Drosten,

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Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of infectious diseases

VOLUME: 196 Suppl 2

Page Numbers: S199-204

Journal Abbreviation: J. Infect. Dis.

ISSN: 0022-1899

DAY: 15

MONTH: Nov

YEAR: 2007

Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories. Information

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LANGUAGE: eng

NlmUniqueID: 413675

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Grant and Affiliation Information for Diagnostic Reverse-Transcription Polymerase Chain Reaction Kit for Filoviruses Based on the Strain Collections of all European Biosafety Level 4 Laboratories.

AFFILIATION: Bernhard-Nocht Institute for Tropical Medicine, Hamburg, 20359, Germany. drosten@bni-hamburg.de.

Country: United States

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MEDLINETA: J Infect Dis

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