Diagnosis of typhoid fever by Dot enzyme immunoassay in an endemic region.

Diagnosis of typhoid fever by Dot enzyme immunoassay in an endemic region. Research Abstract Details 

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Diagnosis of typhoid fever by Dot enzyme immunoassay in an endemic region. Abstract Text:

E Khan,I Azam,S Ahmed,R Hassan,

OBJECTIVE: To compare the results of Dot Enzyme Immunoassay (EIA) and concomitantly performed blood culture for the diagnosis of typhoid fever. METHODS: The results of Dot Enzyme Immunoassay (EIA) of patients who also had blood cultures/bone marrow culture performed at the same time were analyzed. The period of study spans between April 1997-September 1997. RESULTS: A total of 1760 Dot Enzyme Immunoassays were performed from April 1997 to September 1997. Of these, only 128 (7.2%) cases had concomitantly performed blood/bone marrow cultures. Therefore, the study was narrowed down to these 128 cases. Sixty-nine cases were culture positive for S. typhi. Of these culture proven cases, IgM antibodies by Dot EIA were positive only in 49 (71%) cases while 20 (28.9%) of culture proven cases remained negative for IgM antibodies. CONCLUSION: Taking blood/bone marrow culture as gold standard, the sensitivity of Dot EIA was found to be 71% while specificity was found to be 43%. The predictive value of the positive test was found to be 59.7% and that of a negative test was found to be 56.5%.

Diagnosis of typhoid fever by Dot enzyme immunoassay in an endemic region. Publishing Authors By Initials

E Khan,I Azam,S Ahmed,R Hassan,

For similar bacterial infections and mycoses: bacterial infections: gram-negative bacterial infections: enterobacteriaceae infections: salmonella infections: typhoid fever research abstracts see: bacterial infections and mycoses: bacterial infections: gram-negative bacterial infections: enterobacteriaceae infections: salmonella infections: typhoid fever research

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Diagnosis of typhoid fever by Dot enzyme immunoassay in an endemic region. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: JPMA. The Journal of the Pakistan Medical Associat

VOLUME: 52

Page Numbers: 415-7

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ISSN: 0030-9982

DAY: 12

MONTH: Sep

YEAR: 2002

Diagnosis of typhoid fever by Dot enzyme immunoassay in an endemic region. Information

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LANGUAGE: eng

NlmUniqueID: 7501162

Diagnosis of typhoid fever by Dot enzyme immunoassay in an endemic region. Keywords Mesh Terms:

KEYWORDS: Typhoid Fever

MESH TERMS: epidemiology

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Grant and Affiliation Information for Diagnosis of typhoid fever by Dot enzyme immunoassay in an endemic region.

AFFILIATION: Departments of Pathology and Community Health Sciences, Aga Khan University Hospital, Karachi.

Country: Pakistan

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MEDLINETA: J Pak Med Assoc

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