Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction.

Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction. Research Abstract Details 

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Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction. Abstract Text:

Qing-Rong Chen,Gordon Vansant,Kahuku Oades,Maria Pickering,Jun S Wei,Young K Song,Joseph Monforte,Javed Khan,

The small round blue cell tumors of childhood, which include neuroblastoma, rhabdomyosarcoma, non-Hodgkin's lymphoma, and the Ewing's family of tumors, are so called because of their similar appearance on routine histology. Using cDNA microarray gene expression profiles and artificial neural networks (ANNs), we previously identified 93 genes capable of diagnosing these cancers. Using a subset of these, together with some additional genes (total 39), we developed a multiplex polymerase chain reaction (PCR) assay to diagnose these cancer types. Blinded testing of 96 new samples (26 Ewing's family of tumors, 29 rhabdomyosarcomas, 24 neuroblastomas, and 17 lymphomas) using ANNs in a complete leave-one-out analysis demonstrated that all except one sample were accurately diagnosed as their respective category. Moreover, using an ANN-based gene minimization strategy in a separate analysis, we found that the top 31 genes could correctly diagnose all 96 tumors. Our results suggest that this molecular test based on a multiplex PCR reaction may assist the physician in the rapid confirmation of the diagnosis of these cancers.

Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction. Publishing Authors By Initials

QR Chen,G Vansant,K Oades,M Pickering,JS Wei,YK Song,J Monforte,J Khan,

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Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of molecular diagnostics : JMD

VOLUME: 9

Page Numbers: 80-8

Journal Abbreviation:

ISSN: 1525-1578

DAY: 3

MONTH: Feb

YEAR: 2007

Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100893612

Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction. Keywords Mesh Terms:

KEYWORDS: Sarcoma, Ewing's

MESH TERMS: genetics

Chemical & Substance for Abstract: Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction. Information

Substance Name: DNA Primers

Registry Number: 0

Grant and Affiliation Information for Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction.

AFFILIATION: Oncogenomics Section, Pediatric Oncology Branch, Advanced Technology Center, National Cancer Institute, 8717 Grovemont Circle, Gaithersburg, MD 20877, USA.

Country: United States

AGENCY: United States NCI

GRANT: N01-CO-12400

ACRONYM: CO

MEDLINETA: J Mol Diagn

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