Diagnosis of dermatitis herpetiformis by an avidin-biotin-peroxidase method.

Diagnosis of dermatitis herpetiformis by an avidin-biotin-peroxidase method. Research Abstract Details 

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Diagnosis of dermatitis herpetiformis by an avidin-biotin-peroxidase method. Abstract Text:

BACKGROUND AND DESIGN: Immunofluorescence detection of stippled IgA in dermal papillae has been considered the gold standard in the diagnosis of dermatitis herpetiformis (DH). We have developed an immunohistochemical technique using the avidin-biotin-peroxidase complex that is equally effective as direct immunofluorescence in detecting IgA. We retrospectively studied 43 paraffin-embedded biopsy specimens obtained from patients with DH and a variety of other diseases for the presence of IgA along the basement membrane zone. RESULTS: Eleven immunofluorescence-proved cases of DH were found positive for IgA with the avidin-biotin-peroxidase method. One biopsy specimen originally classified as DH was identified and reclassified as linear IgA bullous disease based on the immunoperoxidase findings. All the samples that were positive on direct immunofluorescence were positive with the avidin-biotin-peroxidase method. Control samples of bullous pemphigoid, discoid lupus erythematosus, pemphigus vulgaris, and dermatitis were all negative for IgA deposition. CONCLUSION: The diagnosis of DH on formalin-fixed tissue is possible with the use of an avidin-biotin-peroxidase method, which is convenient and cost-effective.

Diagnosis of dermatitis herpetiformis by an avidin-biotin-peroxidase method. Publishing Authors By Initials

For similar skin and connective tissue diseases: skin diseases: skin diseases, vesiculobullous research abstracts see: skin and connective tissue diseases: skin diseases: skin diseases, vesiculobullous research

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Diagnosis of dermatitis herpetiformis by an avidin-biotin-peroxidase method. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Archives of dermatology

VOLUME: 131

Page Numbers: 571-3

Journal Abbreviation: Arch Dermatol

ISSN: 0003-987X

DAY: 17

MONTH: May

YEAR: 1995

Diagnosis of dermatitis herpetiformis by an avidin-biotin-peroxidase method. Information

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LANGUAGE: eng

NlmUniqueID: 372433

Diagnosis of dermatitis herpetiformis by an avidin-biotin-peroxidase method. Keywords Mesh Terms:

KEYWORDS: Skin Diseases, Vesiculobullous

MESH TERMS: pathology

Chemical & Substance for Abstract: Diagnosis of dermatitis herpetiformis by an avidin-biotin-peroxidase method. Information

Substance Name: Biotin

Registry Number: 58-85-5

Grant and Affiliation Information for Diagnosis of dermatitis herpetiformis by an avidin-biotin-peroxidase method.

AFFILIATION: Department of Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey, USA.

Country: UNITED STATES

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MEDLINETA: Arch Dermatol

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