Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry.

Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry. Research Abstract Details 

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Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry. Abstract Text:

Ronald G Tilton,Sigmund J Haidacher,Wanda S Lejeune,Xiaoquan Zhang,Yingxin Zhao,Alexander Kurosky,Allan R Brasier,Larry Denner,

To understand the spectrum of proteins affected by diabetes and to characterize molecular functions and biological processes they control, we analyzed the renal cortical proteome of db/db mice using 2-DE combined with MALDI-TOF, MALDI-TOF/TOF, and LC-MS/MS. This approach yielded 278 high confidence identifications whose expression levels were significantly increased or decreased >two-fold by diabetes, of which 170 mapped to gene identifiers representing 147 nonredundant proteins. Gene Ontology classification demonstrated that 80% of these proteins modulated physiological functions, 55% involved metabolism, approximately 25% involved carboxylic and organic acid metabolism, 14% involved biosynthesis or catabolism, and 12% involved fatty acid metabolism. Predominant molecular functions were catalytic (61%), oxidoreductase (20%), and transferase (17%) activities, and nucleotide and ATP binding (11-15%). Twenty eight percent of the proteins identified as significantly altered by diabetes were mitochondrial proteins. The top-ranked network described by Ingenuity Pathway Analysis indicated PPARalpha was the most common node of interaction for the numerous enzymes whose expression levels were influenced by diabetes. These differentially regulated proteins create a foundation for a systems biology exploration of molecular mechanisms underlying the pathophysiology of diabetic nephropathy.

Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry. Publishing Authors By Initials

RG Tilton,SJ Haidacher,WS Lejeune,X Zhang,Y Zhao,A Kurosky,AR Brasier,L Denner,

For similar proteins: proteome research abstracts see: proteins: proteome research

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Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proteomics

VOLUME: 7

Page Numbers: 1729-42

Journal Abbreviation: Proteomics

ISSN: 1615-9853

DAY: 3

MONTH: May

YEAR: 2007

Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry. Information

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LANGUAGE: eng

NlmUniqueID: 101092707

Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry. Keywords Mesh Terms:

KEYWORDS: Proteome

MESH TERMS: analysis

Chemical & Substance for Abstract: Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry. Information

Substance Name: Proteome

Registry Number: 0

Grant and Affiliation Information for Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry.

AFFILIATION: Department of Internal Medicine, The University of Texas Medical Branch, Galveston, TX 77555, USA. rgtilton@utmb.edu

Country: Germany

AGENCY: United States NHLBI

GRANT: N01-HV-2814

ACRONYM: HV

MEDLINETA: Proteomics

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