Developmental profiles of phosphorylated and unphosphorylated CREBs in murine calvarial MC3T3-E1 cells.

Developmental profiles of phosphorylated and unphosphorylated CREBs in murine calvarial MC3T3-E1 cells. Research Abstract Details 

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Developmental profiles of phosphorylated and unphosphorylated CREBs in murine calvarial MC3T3-E1 cells. Abstract Text:

M K Sakamoto,K Suzuki,S Takiya,Y Yoshimura,T Imai,A Matsumoto,S Nakamura,

The cAMP-responsive element (CRE) binding protein/activating transcription factor (CREB/ATF) family plays a major role in the expression of skeletal-specific genes and skeletal tissue development. We analyzed the changes of the amount, degree of phosphorylation and binding activity of the CREB/ATF family in the course of development of the murine calvarial osteoblastic cell line MC3T3-E1 as an in vitro model system of bone formation. The amount of CREB in the whole-cell extract detectable by Western blot analysis was high through all stages of development and maximal in the proliferation stage. The degree of phosphorylation estimated with anti-phosphorylated CREB antibody changed greatly and reached high levels in the proliferation stage and early mineralization stage. The ratio of phosphorylated CREB to total CREB in the CREB-CRE complex was also examined by gel shift assay. Although the binding to the consensus/CRE probe reached almost equally high levels in the proliferation stage and early mineralization stage, the relative level of phosphorylated CREB in the CREB-CRE complex was different in these two stages. In the early mineralization stage, most CREB bound to consensus/CRE was phosphorylated, while both phosphorylated and unphosphorylated CREB were bound to consensus/CRE in the proliferation stage. ATF-1 was also detected as a minor component bound to the consensus/CRE probe. The alteration of the binding of CREB to consensus/CRE over the course of osteoblast development supports the hypothesis that CREB may regulate the expression of genes defining the developmental sequence of MC3T3-E1 cells.

Developmental profiles of phosphorylated and unphosphorylated CREBs in murine calvarial MC3T3-E1 cells. Publishing Authors By Initials

MK Sakamoto,K Suzuki,S Takiya,Y Yoshimura,T Imai,A Matsumoto,S Nakamura,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Developmental profiles of phosphorylated and unphosphorylated CREBs in murine calvarial MC3T3-E1 cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 123

Page Numbers: 399-407

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1998

Developmental profiles of phosphorylated and unphosphorylated CREBs in murine calvarial MC3T3-E1 cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Developmental profiles of phosphorylated and unphosphorylated CREBs in murine calvarial MC3T3-E1 cells. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Developmental profiles of phosphorylated and unphosphorylated CREBs in murine calvarial MC3T3-E1 cells. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Developmental profiles of phosphorylated and unphosphorylated CREBs in murine calvarial MC3T3-E1 cells.

AFFILIATION: Department of Orthodontics Hokkaido University, Kita 10, Nishi 8, Kita-ku, Sapporo 060-0813, Japan. saka@den.hokudai.ac.jp

Country: JAPAN

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MEDLINETA: J Biochem

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