Development of transgenic rice seed accumulating a major Japanese cedar pollen allergen (Cry j 1) structurally disrupted for oral immunotherapy.

Development of transgenic rice seed accumulating a major Japanese cedar pollen allergen (Cry j 1) structurally disrupted for oral immunotherapy. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Development of transgenic rice seed accumulating a major Japanese cedar pollen allergen (Cry j 1) structurally disrupted for oral immunotherapy. Abstract Text:

Lijun Yang,Kazuya Suzuki,Sakiko Hirose,Yuhya Wakasa,Fumio Takaiwa,Lijun Yang,Kazuya Suzuki,Sakiko Hirose,Yuhya Wakasa,Fumio Takaiwa,Lijun Yang,Kazuya Suzuki,Sakiko Hirose,Yuhya Wakasa,Fumio Takaiwa,

Rice seed-based edible vaccines expressing T-cell epitope peptides derived from Japanese cedar major pollen allergens have been used to successfully suppress allergen-specific Th2-mediated immunoglobulin E (IgE) responses in mouse experiments. In order to further expand the application of seed-based allergen-specific immunotherapy for controlling Japanese cedar pollinosis, we generated transgenic rice plants that specifically express recombinant Cry j 1 allergens in seeds. Cry j 1 allergens give low specific IgE-binding activity but contain all of the T-cell epitopes. The allergens were expressed directly or as a protein fusion with the major rice storage protein glutelin. Fusion proteins expressed under the control of the strong rice endosperm-specific GluB-1 promoter accumulated in rice endosperm tissue up to 15% of total seed protein. The fusion proteins aggregated with cysteine-rich prolamin and were deposited in endoplasmic reticulum-derived protein body I. The production of transgenic rice expressing structurally disrupted Cry j 1 peptides with low IgE binding activity but spanning the entire Cry j1 region can be used as a universal, safe and effective tolerogen for rice seed-based oral immunotherapy for cedar pollen allergy in humans and other mammals.

Development of transgenic rice seed accumulating a major Japanese cedar pollen allergen (Cry j 1) structurally disrupted for oral immunotherapy. Publishing Authors By Initials

L Yang,K Suzuki,S Hirose,Y Wakasa,F Takaiwa,L Yang,K Suzuki,S Hirose,Y Wakasa,F Takaiwa,L Yang,K Suzuki,S Hirose,Y Wakasa,F Takaiwa,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Development of transgenic rice seed accumulating a major Japanese cedar pollen allergen (Cry j 1) structurally disrupted for oral immunotherapy. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Plant biotechnology journal

VOLUME: 5

Page Numbers: 815-26

Journal Abbreviation: Plant Biotechnol. J.

ISSN: 1467-7652

DAY: 22

MONTH: 08

YEAR: 2007

Development of transgenic rice seed accumulating a major Japanese cedar pollen allergen (Cry j 1) structurally disrupted for oral immunotherapy. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101201889

Development of transgenic rice seed accumulating a major Japanese cedar pollen allergen (Cry j 1) structurally disrupted for oral immunotherapy. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Development of transgenic rice seed accumulating a major Japanese cedar pollen allergen (Cry j 1) structurally disrupted for oral immunotherapy. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Development of transgenic rice seed accumulating a major Japanese cedar pollen allergen (Cry j 1) structurally disrupted for oral immunotherapy.

AFFILIATION: Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Plant Biotechnol J

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Development of transgenic rice seed accumulating a major Japanese cedar pollen allergen Cry j 1 structurally disrupted for oral immunotherapy Related Publications

• Agrobacterium-mediated transformation of a low glutelin mutant of 'Koshihikari' rice variety using the mutated-acetolactate synthase gene derived from rice genome as a selectable marker.
• Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene.
• Biopharming to increase bioactive peptides in rice seed.
• C-terminal extension of rice glutamate decarboxylase (OsGAD2) functions as an autoinhibitory domain and overexpression of a truncated mutant results in the accumulation of extremely high levels of GABA in plant cells.
• Comparison of promoters in transgenic rice.
• Development of transgenic rice seed accumulating a major Japanese cedar pollen allergen (Cry j 1) structurally disrupted for oral immunotherapy.
• Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy.