Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures.

Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures. Research Abstract Details 

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Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures. Abstract Text:

Marion Kennedy,Sunita L D'Souza,Macarena Lynch-Kattman,Staci Schwantz,Gordon Keller,

The onset of hematopoiesis in the mouse embryo and in the embryonic stem (ES) cell differentiation model is defined by the emergence of the hemangioblast, a progenitor with both hematopoietic and vascular potential. While there is evidence for the existence of a hemangioblast in the mouse, it is unclear if this progenitor develops during the establishment of the human hematopoietic system. In this report, we have mapped hematopoietic development in human ES cell (hESC) differentiation cultures and demonstrated that a comparable hemangioblast population exists. The human hemangioblasts were identified by their capacity to generate blast colonies that display both hematopoietic and vascular potential. These colony-forming cells express the receptor tyrosine kinase KDR (VEGF receptor 2) and represent a transient population that develops in BMP-4-stimulated embryoid bodies (EBs) between 72 and 96 hours of differentiation, prior to the onset of the primitive erythroid program. Two distinct types of hemangioblasts were identified, those that give rise to primitive erythroid cells, macrophages, and endothelial cells and those that generate only the primitive erythroid population and endothelial cells. These findings demonstrate for the first time the existence of the human hemangioblast and in doing so identify the earliest stage of hematopoietic commitment.

Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures. Publishing Authors By Initials

M Kennedy,SL D'Souza,M Lynch-Kattman,S Schwantz,G Keller,

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Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Blood

VOLUME: 109

Page Numbers: 2679-87

Journal Abbreviation: Blood

ISSN: 0006-4971

DAY: 1

MONTH: Apr

YEAR: 2007

Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures. Information

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LANGUAGE: eng

NlmUniqueID: 7603509

Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures. Keywords Mesh Terms:

KEYWORDS: Vascular Endothelial Growth Factor Recep

MESH TERMS: genetics

Chemical & Substance for Abstract: Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures. Information

Substance Name: Vascular Endothelial Growth Factor Recep

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures.

AFFILIATION: Department of Gene and Cell Medicine, The Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York. NY 10029, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL080627

ACRONYM: HL

MEDLINETA: Blood

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