Development of quantitative real-time PCR assays to detect Rickettsia typhi and Rickettsia felis, the causative agents of murine typhus and flea-borne spotted fever.

Development of quantitative real-time PCR assays to detect Rickettsia typhi and Rickettsia felis, the causative agents of murine typhus and flea-borne spotted fever. Research Abstract Details 

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Development of quantitative real-time PCR assays to detect Rickettsia typhi and Rickettsia felis, the causative agents of murine typhus and flea-borne spotted fever. Abstract Text:

Katherine M Henry,Ju Jiang,Patrick J Rozmajzl,Abdu F Azad,Kevin R Macaluso,Allen L Richards,

Rickettsia typhi and Rickettsia felis are the etiologic agents of murine typhus and flea-borne spotted fever, respectively. We have constructed two quantitative real-time polymerase chain reaction (qPCR) assays to detect these pathogenic rickettsiae. The qPCR assays were developed utilizing unique sequences of the R. typhi and R. felis outer membrane protein B genes (ompB) to design the specific primers and molecular beacon probes. The assays were found to be species-specific and did not yield false-positive reactions with nucleic acid from other rickettsiae, orientiae, neorickettsiae or unrelated bacteria. In addition, the assays were sensitive enough to detect three target sequence copies per reaction and were capable of detecting R. typhi and R. felis nucleic acid in the cat flea, Ctenocephalides felis. These results demonstrate that two sensitive and specific qPCR assays have been successfully developed to detect and enumerate R. typhi and R. felis.

Development of quantitative real-time PCR assays to detect Rickettsia typhi and Rickettsia felis, the causative agents of murine typhus and flea-borne spotted fever. Publishing Authors By Initials

KM Henry,J Jiang,PJ Rozmajzl,AF Azad,KR Macaluso,AL Richards,

For similar bacterial infections and mycoses: bacterial infections: gram-negative bacterial infections: rickettsiaceae infections: rickettsia infections: typhus, endemic flea-borne research abstracts see: bacterial infections and mycoses: bacterial infections: gram-negative bacterial infections: rickettsiaceae infections: rickettsia infections: typhus, endemic flea-borne research

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Development of quantitative real-time PCR assays to detect Rickettsia typhi and Rickettsia felis, the causative agents of murine typhus and flea-borne spotted fever. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular and cellular probes

VOLUME: 21

Page Numbers: 17-23

Journal Abbreviation: Mol. Cell. Probes

ISSN: 0890-8508

DAY: 1

MONTH: 07

YEAR: 2006

Development of quantitative real-time PCR assays to detect Rickettsia typhi and Rickettsia felis, the causative agents of murine typhus and flea-borne spotted fever. Information

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LANGUAGE: eng

NlmUniqueID: 8709751

Development of quantitative real-time PCR assays to detect Rickettsia typhi and Rickettsia felis, the causative agents of murine typhus and flea-borne spotted fever. Keywords Mesh Terms:

KEYWORDS: Typhus, Endemic Flea-Borne

MESH TERMS: microbiology

Chemical & Substance for Abstract: Development of quantitative real-time PCR assays to detect Rickettsia typhi and Rickettsia felis, the causative agents of murine typhus and flea-borne spotted fever. Information

Substance Name: DNA, Bacterial

Registry Number: 0

Grant and Affiliation Information for Development of quantitative real-time PCR assays to detect Rickettsia typhi and Rickettsia felis, the causative agents of murine typhus and flea-borne spotted fever.

AFFILIATION: Rickettsial Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500, USA.

Country: England

AGENCY: United States NIAID

GRANT: R37 AI17828

ACRONYM: AI

MEDLINETA: Mol Cell Probes

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