Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection.

Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection. Research Abstract Details 

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Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection. Abstract Text:

Zengyan Zhang,Jingsheng Xu,Qinjuan Xu,Philip Larkin,Zhiyong Xin,

The distal segment of the long arm of the Thinopyrum intermedium chromosome 7Ai1 carries the barley yellow dwarf virus (BYDV) resistance gene Bdv2. This segment was transferred to the distal region of the long arm of wheat chromosome 7D in the Yw series of translocation lines by using the ph1b mutant to induce homoeologous pairing. To transfer Bdv2 to commercial varieties, we developed two resistance gene-analog polymorphism (RGAP) markers, Tgp-1(350) and Tgp-2(210), and one randomly amplified polymorphic DNA (RAPD) marker, OPD04(1300). The diagnostic fragments of the RGAP marker Tgp-1(350) and the RAPD marker OPD04(1300) were cloned, sequenced and converted into sequence-characterized amplified region (SCAR) markers, named SC-gp1 and SC-D04, respectively. SC-gp1 and SC-D04 were validated based on available translocation lines and segregating F(2) individuals. Our results indicated that the SCAR markers co-segregated with the BYDV resistance associated with Bdv2. Therefore, they can be used as a low-cost, high-throughput alternative to conventional phenotypic screening in wheat-breeding programs exploiting Bdv2. The marker-assisted selection for BYDV resistance was successfully performed in a wheat-breeding program.

Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection. Publishing Authors By Initials

Z Zhang,J Xu,Q Xu,P Larkin,Z Xin,

For similar plants: plant families and groups: angiosperms: poaceae: triticum research abstracts see: plants: plant families and groups: angiosperms: poaceae: triticum research

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Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 109

Page Numbers: 433-9

Journal Abbreviation:

ISSN: 0040-5752

DAY: 6

MONTH: 04

YEAR: 2004

Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection. Information

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LANGUAGE: eng

NlmUniqueID: 145600

Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection. Keywords Mesh Terms:

KEYWORDS: Triticum

MESH TERMS: virology

Chemical & Substance for Abstract: Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection. Information

Substance Name: Genetic Markers

Registry Number: 0

Grant and Affiliation Information for Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection.

AFFILIATION: Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture, Institute of Crop Breeding and Cultivation, Chinese Academy of Agriculture Sciences, Beijing 100081, China.

Country: Germany

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MEDLINETA: Theor Appl Genet

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