[Development of monoclonal antibody against fumonisin B1 and ELISA-kit of quantitative analysis for fumonisin B1]

[Development of monoclonal antibody against fumonisin B1 and ELISA-kit of quantitative analysis for fumonisin B1] Research Abstract Details 

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[Development of monoclonal antibody against fumonisin B1 and ELISA-kit of quantitative analysis for fumonisin B1] Abstract Text:

Tao Jiang,Hui-zhi Gong,Feng-qin Li,Rong Ji,

OBJECTIVE: To detect the contamination level of fumonisin B1 in grain, and to develop rapid detection kit that possess patent of China. METHODS: Hybridoma cell line excreting monoclonal antibody against fumonisin B, was produced using B cell hybridoma technique and develop a rapid, sensitive, quantitative ELISA-kit for detection fumonisin B1. RESULTS: The monoclonal antibody used in the ELISA-kit were tested for subtype as IgG1 and its affinity constant was 8.3 x 10(-8) mol/L. The monoclonal antibody obtained in the present study was highly specific to fumonisin B1 , because no cross reactions between the monoclonal antibodies against fumonisin B1 with the analogues of fumonisin B, were found. The limited concentration of the ELISA-kit was 5 ng/ml, linear range was 50-500 ng/ml, the linear equation was Y = -0.582 x +1.793( = 0.99, P < 0.05). The recovery rate of maize on the level of 50n g/ml, 200 ng/ml and 500 ng/ml was among 71.89%-112.95%. The kit can be stored at normal tempertature in ten months at least. The coefficient of variant winthin-laboratory and between-laboratory was less than 20%.

[Development of monoclonal antibody against fumonisin B1 and ELISA-kit of quantitative analysis for fumonisin B1] Publishing Authors By Initials

T Jiang,HZ Gong,FQ Li,R Ji,

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[Development of monoclonal antibody against fumonisin B1 and ELISA-kit of quantitative analysis for fumonisin B1] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Wei sheng yan jiu = Journal of hygiene research

VOLUME: 35

Page Numbers: 209-12

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ISSN: 1000-8020

DAY: 8

MONTH: Mar

YEAR: 2006

[Development of monoclonal antibody against fumonisin B1 and ELISA-kit of quantitative analysis for fumonisin B1] Information

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LANGUAGE: chi

NlmUniqueID: 9426367

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AFFILIATION: Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.

Country: China

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MEDLINETA: Wei Sheng Yan Jiu

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