Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin b1 in grain and feed samples.

Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin b1 in grain and feed samples. Research Abstract Details 

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Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin b1 in grain and feed samples. Abstract Text:

Won-Bo Shim,Zheng-You Yang,Jung-Sook Kim,Ji-Young Kim,Sung-Jo Kang,Gun-Jo Woo,Young-Chul Chung,Eremin Sergei A,Duck-Hwa Chung,Won-Bo Shim,Zheng-You Yang,Jung-Sook Kim,Ji-Young Kim,Sung-Jo Kang,Gun-Jo Woo,Young-Chul Chung,Sergei A Eremin,Duck-Hwa Chung,

An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.

Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin b1 in grain and feed samples. Publishing Authors By Initials

WB Shim,ZY Yang,JS Kim,JY Kim,SJ Kang,GJ Woo,YC Chung,E Sergei A,DH Chung,WB Shim,ZY Yang,JS Kim,JY Kim,SJ Kang,GJ Woo,YC Chung,SA Eremin,DH Chung,

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Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin b1 in grain and feed samples. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of microbiology and biotechnology

VOLUME: 17

Page Numbers: 1629-37

Journal Abbreviation: J. Microbiol. Biotechnol.

ISSN: 1017-7825

DAY: 24

MONTH: Oct

YEAR: 2007

Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin b1 in grain and feed samples. Information

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LANGUAGE: eng

NlmUniqueID: 9431852

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AFFILIATION: Divison of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University, 119992 Moscow, Russia.

Country: Korea (South)

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MEDLINETA: J Microbiol Biotechnol

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