[Development of ELISA-kit of quantitative analysis for Zearalenone]

[Development of ELISA-kit of quantitative analysis for Zearalenone] Research Abstract Details 

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[Development of ELISA-kit of quantitative analysis for Zearalenone] Abstract Text:

Yu-ping Wang,Rong Ji,Tao Jiang,Wei-jun Kang,

OBJECTIVE: To develope a rapid, sensitive, quantitative ELISA-kit for Zearalenone and determine zearalenone in cereals. METHODS: On the base of monoclonal antibodies against ZEN, apply indirect ELISA to study the performance parameter of the kit. RESULTS: The limited concentration of detection of the ELISA-kit was 1ng/ml, linear range was 1-200 ng/ml, the linear equation was Y = 0.99 - 0.40 x (R2 = 0.99). The inhibition concentration of 50% against ZEN was 16.3 ng/ml. The average recovery rate of spiked corn and wheat was 96.5% and 95.5%, respectively, the coefficient of variant was 13.2% and 10.9%, respectively. The kit can be stored at 4 degrees C over 6 months. The cross reaction rate with the other mycotoxins was less than 1%, and coefficient of variant within-laboratory and between-laboratory was less than 15% and less than 20%, respectively. Detecting the VICAM sample with ELISA method and HPLC method, the results were within the range of the sample, and there was no statistic difference between the two methods. CONCLUSION: This ELISA-kit was quick, sensitive, stable and specific and can be used to determine ZEN in cereals.

[Development of ELISA-kit of quantitative analysis for Zearalenone] Publishing Authors By Initials

YP Wang,R Ji,T Jiang,WJ Kang,

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[Development of ELISA-kit of quantitative analysis for Zearalenone] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Wei sheng yan jiu = Journal of hygiene research

VOLUME: 35

Page Numbers: 221-4

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ISSN: 1000-8020

DAY: 8

MONTH: Mar

YEAR: 2006

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LANGUAGE: chi

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AFFILIATION: Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.

Country: China

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MEDLINETA: Wei Sheng Yan Jiu

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