Development of electrochemical biosensors based on sol-gel enzyme encapsulation and protective polymer membranes.

Development of electrochemical biosensors based on sol-gel enzyme encapsulation and protective polymer membranes. Research Abstract Details 

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Development of electrochemical biosensors based on sol-gel enzyme encapsulation and protective polymer membranes. Abstract Text:

Rasa Pauliukaite,Monika Schoenleber,Pankaj Vadgama,Christopher M A Brett,Rasa Pauliukaite,Monika Schoenleber,Pankaj Vadgama,Christopher M A Brett,Rasa Pauliukaite,Monika Schoenleber,Pankaj Vadgama,Christopher M A Brett,

Protective polymer coatings have been used to enhance the retention of enzymes in sol-gel films as immobilisation phases in electrochemical biosensors. Carbon film electrodes were electrochemically modified with poly(neutral red) (PNR). These electrodes were coated with oxysilane sol-gels incorporating glucose oxidase and an outer coating of carboxylated PVC (CPVC) or polyurethane (PU), with and without Aliquat-336 or isopropyl myristate (IPM) plasticizer, was applied. The biosensors were characterised electrochemically using cyclic voltammetry and amperometry, electrochemical impedance spectroscopy and scanning electron microscopy. Impedance spectra showed that the electrode surface is most active when the sol-gel-GOx layer is not covered with a membrane. However, membranes without plasticizer extend the lifetime of the biosensor to more than 2 months when PU is used as an outer membrane. The linear range of the biosensors was found to be 0.05-0.50 mM of glucose and the biosensor with PU outer membrane exhibited higher sensitivity (ca.117 nA mM(-1)) in the region of linear response than that with CPVC. The biosensors were applied to glucose measurement in natural samples of commercial orange juice.

Development of electrochemical biosensors based on sol-gel enzyme encapsulation and protective polymer membranes. Publishing Authors By Initials

R Pauliukaite,M Schoenleber,P Vadgama,CM Brett,R Pauliukaite,M Schoenleber,P Vadgama,CM Brett,R Pauliukaite,M Schoenleber,P Vadgama,CM Brett,

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Development of electrochemical biosensors based on sol-gel enzyme encapsulation and protective polymer membranes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical and bioanalytical chemistry

VOLUME: 390

Page Numbers: 1121-31

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ISSN: 1618-2650

DAY: 16

MONTH: 12

YEAR: 2007

Development of electrochemical biosensors based on sol-gel enzyme encapsulation and protective polymer membranes. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Development of electrochemical biosensors based on sol-gel enzyme encapsulation and protective polymer membranes.

AFFILIATION: Departamento de Química, Universidade de Coimbra, 3004-535, Coimbra, Portugal.

Country: Germany

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MEDLINETA: Anal Bioanal Chem

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