Development of des-gamma-carboxy prothrombin (DCP) measuring reagent using the LiBASys clinical analyzer.

Development of des-gamma-carboxy prothrombin (DCP) measuring reagent using the LiBASys clinical analyzer. Research Abstract Details 

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Development of des-gamma-carboxy prothrombin (DCP) measuring reagent using the LiBASys clinical analyzer. Abstract Text:

Abstract Background: Des-gamma-carboxy prothrombin (DCP), Lens culinaris agglutinin-reactive alpha-fetoprotein ratio (AFP-L3) and total alpha-fetoprotein (AFP) are tumor markers useful for diagnosing and determining the prognosis of hepatocellular carcinoma (HCC). There is a real need for measurement of these three markers on a one-assay platform. Methods: A method of DCP measurement in human serum was developed using liquid binding assay (LBA), which enables rapid antigen-antibody reaction and bound/free separation on the LiBASys clinical analyzer. Results: The dilution curve for DCP was linear up to 500 ng/mL. The limit of detection of DCP concentration was 0.5 ng/mL. Intra- and inter-assay coefficients of variation of DCP were 0.7%-2.4% and 2.2%-6.5%, respectively. This method was free from interference by hemoglobin, bilirubin, ditaurobilirubin, intrafat, ascorbate, galactose, glucose and rheumatoid factor. The analytical recoveries of DCP added to serum were 91.7%-108.2%. DCP concentration measured with the LBA method was linear and was significantly correlated with that measured with the ELISA method. Conclusions: The LiBASys clinical analyzer made possible measurement of the complementary tumor markers, HCC, total AFP, AFP-L3 and DCP. Clin Chem Lab Med 2008;46:411-6.

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Development of des-gamma-carboxy prothrombin (DCP) measuring reagent using the LiBASys clinical analyzer. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Clinical chemistry and laboratory medicine : CCLM

VOLUME: 46

Page Numbers: 411-6

Journal Abbreviation: Clin. Chem. Lab. Med.

ISSN: 1434-6621

DAY: 28

MONTH: 02

YEAR: 2008

Development of des-gamma-carboxy prothrombin (DCP) measuring reagent using the LiBASys clinical analyzer. Information

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LANGUAGE: eng

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AFFILIATION: 1New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries Ltd., Hyogo, Japan.

Country: Germany

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MEDLINETA: Clin Chem Lab Med

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