Development of biodegradable electrospun scaffolds for dermal replacement.

Development of biodegradable electrospun scaffolds for dermal replacement. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Development of biodegradable electrospun scaffolds for dermal replacement. Abstract Text:

Our objective is to develop a synthetic biodegradable replacement dermal substitute for tissue engineering of skin and oral mucosa. Our in vivo criteria were that candidate scaffolds should allow surrounding cells to migrate fully into the scaffolds, enabling vasculogenesis and remodelling without invoking a chronic inflammatory response. We examined a total of six experimental electrospun polymer scaffolds: (1) poly-l-lactide (PLLA); (2) PLLA+10% oligolactide; (3) PLLA+rhodamine and (4-6) three poly(d,l)-lactide-co-glycolide (PLGA) random multiblock copolymers, with decreasing lactide/glycolide mole fractions (85:15, 75:25 and 50:50). These were evaluated for degradation in vitro up to 108 days and in vivo in adult male Wistar rats from 4 weeks to 12 months. In vivo, all scaffolds permitted good cellular penetration, with no adverse inflammatory response outside the scaffold margin and with no capsule formation around the periphery. The breakdown rate for each scaffold in vitro versus in vivo was similar, and an increase in the ratio of polyglycolide to polylactide correlated with an increase in breakdown rate, as expected. Scaffolds of PLLA were stable in vivo even after 12 months whereas scaffolds fabricated from PLGA 85:15 and 75:25 revealed a 50% loss of mass after 4 and 3 months, respectively. In vitro PLGA 85:15 and 75:25 scaffolds were able to support keratinocyte, fibroblast and endothelial cell growth and extracellular matrix production, with evidence of new collagen production after 7 days. In conclusion, the data supports the development of PLGA 85:15 and 75:25 electrospun polymer scaffolds as potential degradable biomaterials for dermal replacement.

Development of biodegradable electrospun scaffolds for dermal replacement. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Development of biodegradable electrospun scaffolds for dermal replacement. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biomaterials

VOLUME: 29

Page Numbers: 3091-104

Journal Abbreviation: Biomaterials

ISSN: 0142-9612

DAY: 29

MONTH: 04

YEAR: 2008

Development of biodegradable electrospun scaffolds for dermal replacement. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8100316

Development of biodegradable electrospun scaffolds for dermal replacement. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Development of biodegradable electrospun scaffolds for dermal replacement. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Development of biodegradable electrospun scaffolds for dermal replacement.

AFFILIATION: Tissue Engineering Group, Department of Engineering Materials, Kroto Research Institute, University of Sheffield, North Campus, Broad Lane, Sheffield S3 7HQ, United Kingdom.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biomaterials

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Development of biodegradable electrospun scaffolds for dermal replacement Related Publications

• Agent based modelling helps in understanding the rules by which fibroblasts support keratinocyte colony formation.
• Alternative methodology for the analysis of progesterone, testosterone, and epi-testosterone in bovine liver and veal muscle.
• An epidemiologic study of aboriginal adolescent risk in Canada: the meaning of suicide.
• An integrated systems biology approach to understanding the rules of keratinocyte colony formation.
• Analysis of streptomycin and dihydrostreptomycin in milk by liquid chromatography.
• Bacterial inhibition tests used to screen for antimicrobial veterinary drug residues in slaughtered animals.
• Committee on drugs and related topics. Drug residues in animal tissues.
• Comparison of four commercially available rapid test kits with liquid chromatography for detecting penicillin G residues in bovine plasma.
• Continued exposure to maternal distress in early life is associated with an increased risk of childhood asthma.
• Deficiency in cytosolic malic enzyme does not increase acetaminophen-induced hepato-toxicity.
• Determination of clopidol residues in chicken tissues by liquid chromatography: collaborative study.
• Determination of eight synthetic pyrethroids in bovine fat by gas chromatography with electron capture detection.
• Determination of neomycin in animal tissues by liquid chromatography.
• Determination of streptomycin and dihydrostreptomycin in animal tissue by on-line sample enrichment liquid chromatography.
• Development of biodegradable electrospun scaffolds for dermal replacement.
• Drug residues in animal tissues.
• Economic aspects of hypercholesterolemia treatment with HMG-CoA reductase inhibitors: a review of recent developments.
• Enhancement of keratinocyte performance in the production of tissue-engineered skin using a low-calcium medium.
• Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells.
• Granuloma of the larynx following endotracheal anaesthesia.
• Influence of sire, sex of fetus and type of pregnancy on conceptus development.
• Journal of burn care & research : official publication of the American Burn Association
• Milk yield of primiparous beef cows from three calving systems and varied weaning ages.
• NPY Y1 and Y5 receptor selective antagonists as anti-obesity drugs.
• Prevalence of cytomegalovirus antibodies in the personnel of a children's hospital, Halifax, Nova Scotia.
• Significant associations of stearoyl-CoA desaturase (SCD1) gene with fat deposition and composition in skeletal muscle.
• Venereal disease work in the public health nursing program.
• Youth-adult partnerships in decision making: disseminating and implementing an innovative idea into established organizations and communities.