Development of Binding Assays for the SH2 Domain of Grb7 and Grb2 Using Fluorescence Polarization.

Development of Binding Assays for the SH2 Domain of Grb7 and Grb2 Using Fluorescence Polarization. Research Abstract Details 

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Development of Binding Assays for the SH2 Domain of Grb7 and Grb2 Using Fluorescence Polarization. Abstract Text:

Jean-Philippe Luzy,Huixiong Chen,Brunilde Gril,Wang-Qing Liu,Michel Vidal,Dominique Perdereau, Burnol,Christiane Garbay,

Adaptor proteins Grb7 and Grb2 have been implicated as being 2 potential therapeutic targets in several human cancers, especially those that overexpress ErbB2. These 2 proteins contain both a SH2 domain (Src homology 2) that binds to phosphorylated tyrosine residues contained within ErbB2 and other specific protein targets. Two assays based on enzyme-linked immunosorbent assay and fluorescence polarization methods have been developed and validated to find and rank inhibitors for both proteins binding to the pY(1139). Fluorescence polarization assays allowed the authors to determine quickly and reproducibly affinities of peptides from low nanomolar to high micromolar range and to compare them directly for Grb7 and Grb2. As a result, the assays have identified a known peptidomimetic Grb2 SH2 inhibitor (mAZ-pTyr-(alphaMe)pTyr-Asn-NH(2)) that exhibits the most potent affinity for the Grb7 SH2 domain described to date.

Development of Binding Assays for the SH2 Domain of Grb7 and Grb2 Using Fluorescence Polarization. Publishing Authors By Initials

JP Luzy,H Chen,B Gril,WQ Liu,M Vidal,D Perdereau,AF Burnol,C Garbay,

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Development of Binding Assays for the SH2 Domain of Grb7 and Grb2 Using Fluorescence Polarization. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomolecular screening : the official j

VOLUME: 13

Page Numbers: 112-9

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ISSN: 1087-0571

DAY: 23

MONTH: 01

YEAR: 2008

Development of Binding Assays for the SH2 Domain of Grb7 and Grb2 Using Fluorescence Polarization. Information

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LANGUAGE: eng

NlmUniqueID: 9612112

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Grant and Affiliation Information for Development of Binding Assays for the SH2 Domain of Grb7 and Grb2 Using Fluorescence Polarization.

AFFILIATION: INSERM U648, Laboratoire de Pharmacochimie Moléculaire et Cellulaire, UFR Biomédicale, Université Paris Descartes, Paris, France.

Country: United States

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MEDLINETA: J Biomol Screen

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