Development of astroglial cells in patterned neuronal cultures.

Development of astroglial cells in patterned neuronal cultures. Research Abstract Details 

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Development of astroglial cells in patterned neuronal cultures. Abstract Text:

Yoonkey Nam,Gregory J Brewer,Bruce C Wheeler,Yoonkey Nam,Gregory J Brewer,Bruce C Wheeler,

The purpose of this work was to study the development of astroglial cells in patterned neuronal cultures. Hippocampal neurons, derived from embryonic stage (E18) rats and cultured in serum-free Neurobasal/B27 medium, grew to follow patterns of poly(D-lysine) created by micro-contact printing. The growth of the astroglial cells and the co-localization of neurons and astroglial cells were measured for up to one month using fluorescence immunostaining of neurons and astroglial cells. Neurons grew to form square patterns within 2 weeks, while astroglia only started to emerge in the same period. Astroglial cells continued to proliferate for a month following a general growth curve. Over 90% of the astroglial cell area co-localized with neurons (within 2 mum) at an early stage of astroglial development (13 DIV). Over the remaining period, astroglial cells proliferated and the co-localization was 80%. Hence, in these culture conditions astroglial cells develop 2-3 weeks later than neurons but remain highly co-localized with neurons.

Development of astroglial cells in patterned neuronal cultures. Publishing Authors By Initials

Y Nam,GJ Brewer,BC Wheeler,Y Nam,GJ Brewer,BC Wheeler,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Development of astroglial cells in patterned neuronal cultures. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 18

Page Numbers: 1091-100

Journal Abbreviation:

ISSN: 0920-5063

DAY: 3

MONTH: 12

YEAR: 2007

Development of astroglial cells in patterned neuronal cultures. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007393

Development of astroglial cells in patterned neuronal cultures. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Development of astroglial cells in patterned neuronal cultures. Information

Substance Name: Culture Media, Serum-Free

Registry Number: 0

Grant and Affiliation Information for Development of astroglial cells in patterned neuronal cultures.

AFFILIATION: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.

Country: Netherlands

AGENCY: United States NIBIB

GRANT: R01 EB000786

ACRONYM: EB

MEDLINETA: J Biomater Sci Polym Ed

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