Development of articular cartilage grafts using organoid formation techniques.

Development of articular cartilage grafts using organoid formation techniques. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Development of articular cartilage grafts using organoid formation techniques. Abstract Text:

Y Irie,H Mizumoto,S Fujino,T Kajiwara,

In order to develop articular cartilage grafts, one must control shape and safety. We have developed scaffold-free culture methods in which the cells form multicellular aggregates (organoids). In this study, we applied the organoid culture method to chondrocytes attempting to reconstitute articular cartilage grafts. Primary rat costal chondrocytes and subcultured human articular chondrocytes were immobilized in hollow fibers by centrifugation at a density of 3 x 10(8) cells/cm(3) to induce the formation of cylindrical-shaped organoids. To improve convenience, we developed a culture device to form sheet-shaped organoids (organoid-sheet). Primary bovine articular chondrocytes were cultured in this device. These organoids were evaluated by histological and gene expression analyses. In the primary rat culture system, chondrocytes formed cylindrical organoids in hollow fibers. Histochemical analysis revealed the presence of extracellular matrix (collagen and proteoglycan). The organoid maintained cartilage-specific gene expression (type II collagen, aggrecan) for 1 month of culture. In the subcultured human chondrocyte system, the organoid regained the decreased cartilage-specific gene expression. In the primary bovine culture system, the cells formed a 300 mum thickness organoid-sheet including abundant extracellular matrix. In conclusion, our organoid formation method was effective to form cartilage-like tissue. This result suggested that the technique may be applicable for the development of an articular cartilage graft.

Development of articular cartilage grafts using organoid formation techniques. Publishing Authors By Initials

Y Irie,H Mizumoto,S Fujino,T Kajiwara,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Development of articular cartilage grafts using organoid formation techniques. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Transplantation proceedings

VOLUME: 40

Page Numbers: 631-3

Journal Abbreviation:

ISSN: 0041-1345

DAY: 31

MONTH: Mar

YEAR: 2008

Development of articular cartilage grafts using organoid formation techniques. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 243532

Development of articular cartilage grafts using organoid formation techniques. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Development of articular cartilage grafts using organoid formation techniques. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Development of articular cartilage grafts using organoid formation techniques.

AFFILIATION:

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Transplant Proc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Development of articular cartilage grafts using organoid formation techniques Related Publications

• A new culture technique for hepatocyte organoid formation and long-term maintenance of liver-specific functions.
• Antithrombin improves fetal condition in women with severe pre-eclampsia before 32 weeks of gestation; a randomized, double-blind, placebo-controlled trial.
• Development of articular cartilage grafts using organoid formation techniques.
• Evaluation of a hybrid artificial liver module with liver lobule-like structure in rats with liver failure.
• Hepatic Differentiation of Embryonic Stem Cells in HF/Organoid Culture.
• Hepatic differentiation of mouse embryonic stem cells in a bioreactor using polyurethane/spheroid culture.
• Hepatic differentiation of mouse embryonic stem cells in a three-dimensional culture system using polyurethane foam.
• Insulinomimetic Zn(II) Complexes as Evaluated by Both Glucose-Uptake Activity and Inhibition of Free Fatty Acids Release in Isolated Rat Adipocytes.
• Investigations of the architecture of tamarind seed polysaccharide in aqueous solution by different scattering techniques.
• Malic acid production by an electrochemical reduction system combined with the use of diaphorase and methylviologen.
• Metabolic pathways leading from amino acids to vitamin B12 in Propionibacterium shermanii, and the sources of the seven methyl carbons.
• On the effect of cobaltic complex salts upon the Yoshida sarcoma. II.
• Proposal and experimental verification of Bragg wavelength distribution measurement within a long-length FBG by synthesis of optical coherence function.
• Re: Prevalence and associated factors of enuresis in Turkish children.
• Reconstruction of cartilage tissue using scaffold-free organoid culture technique.
• Role of central serotonergic (5-HT2) receptor in blood pressure regulation in rats.
• Studies on the scarlet fever toxoid; on the viristatic action of streptococcus toxoid upon vaccinia virus.
• Transient depression of LWa antigen with coincident production of anti-LWa repeated in relapses of malignant lymphoma.
• [Intracoronary thrombolysis in acute myocardial infarction]
• [Questions and answers: a flat, raised lesion of the descending colon]
• [Successful removal of infected pacemaker leads under extracorporeal circulation in three cases]