Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine.

Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine. Abstract Text:

A K Hewavitharana,S Lee,P A Dawson,D Markovich,P N Shaw,

An HPLC-MS/MS method has been developed for the selective quantitative analysis of paracetamol and its two major metabolites. The use of tandem MS enabled the detection and quantitation of metabolites in small sample sizes with high sensitivity and selectivity. Isocratic elution using acetonitrile and water containing formic acid combined with electrospray-tandem MS enabled the separation and accurate quantitation of each analyte and the internal standard 3-acetamidophenol. The on-column limits of detection for paracetamol, paracetamol sulfate, and paracetamol glucuronide were 2.4, 1.2, and 1.2 pmol, respectively. The method was applied to quantitate paracetamol and its metabolites in mouse urine. It is highly specific, sensitive, and easily adaptable to measure these analytes in biological fluids of other animals.

Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine. Publishing Authors By Initials

AK Hewavitharana,S Lee,PA Dawson,D Markovich,PN Shaw,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical biochemistry

VOLUME: 374

Page Numbers: 106-11

Journal Abbreviation: Anal. Biochem.

ISSN: 0003-2697

DAY: 17

MONTH: 11

YEAR: 2007

Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370535

Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine.

AFFILIATION: School of Pharmacy, University of Queensland, Brisbane 4072, Australia.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Anal Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Development of an HPLC-MS/MS method for the selective determination of paracetamol metabolites in mouse urine Related Publications

• A cricket Gene Index: a genomic resource for studying neurobiology, speciation, and molecular evolution.
• Age-associated defect in human TLR-1/2 function.
• Aluminum adjuvant linked to gulf war illness induces motor neuron death in mice.
• Biotransformation of maximakinin, a bradykinin-related nonadecapeptide from toad venom, by mammalian kallikrein and salivary proteases.
• Community exposure to perfluorooctanoate: relationships between serum concentrations and exposure sources.
• Community exposure to perfluorooctanoate: relationships between serum levels and certain health parameters.
• Ct05 mechanical valve or tissue valve: do patients regret their decision?
• Ct23 what's new in ICU.
• Ct25 cpap: an overview and current applications.
• Ct27 a history of errors and the christchurch solution.
• Ct32 when to call the surgeon.
• Developmental divergence of sleep-wake patterns in orexin knockout and wild-type mice.
• Do the predictors of child conduct problems vary by high- and low-levels of socioeconomic and neighborhood risk?
• Ending the splits.
• Essentials of sleep recordings in Drosophila: moving beyond sleep time.
• From Sydney to Athens: preparing for the Olympic and Paralympic Games.
• Identification of a biomarker for sleep drive in flies and humans.
• Impact of neighborhood disadvantage on overt behavior problems during early childhood.
• Leptin predicts the development of diabetes in Mauritian men, but not women: a population-based study.
• Mitochondrial DNA haplogroups and amyotrophic lateral sclerosis.
• Prevaccine determination of the expression of costimulatory B7 molecules in activated monocytes predicts influenza vaccine responses in young and older adults.
• Six year incidence and progression of diabetic retinopathy: results from the Mauritius diabetes complication study.
• Suppression of insulin receptor substrate 1 (IRS-1) promotes mammary tumor metastasis.
• Synthesis and stereochemical assignment of brasilibactin A.
• Synthesis of kaempferitrin.
• Testing mediators of intervention effects in randomized controlled trials: An evaluation of two eating disorder prevention programs.
• The African orphan crisis and international adoption.
• The stimulatory potency of T cell antigens is influenced by the formation of the immunological synapse.
• Ugo1p is a multipass transmembrane protein with a single carrier domain required for mitochondrial fusion.
• [The self-training of a therapist : a demystification process.]