Development of a zona-free hamster ova bioassay for goat sperm.

Development of a zona-free hamster ova bioassay for goat sperm. Research Abstract Details 

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Development of a zona-free hamster ova bioassay for goat sperm. Abstract Text:

T Berger,

In vitro conditions for a zona-free hamster ova bioassay of caprine sperm fertility were assessed. Washing the cryopreserved sperm by dilution and centrifugation resulted in greater ability to penetrate zona-free hamster ova than allowing the sperm to swim-up into medium. A 12-h preincubation of sperm prior to insemination of the zona-free hamster ova was optimal. Sperm had greater ability to penetrate the zona-free hamster ova when incubated in a Tris-buffered medium than when incubated in Ham's F-10 (95 vs 2%, P<0.001), although motility was not well maintained in the Tris-buffered medium. Ten million sperm/ml was sufficient for maximum penetration. The ability to penetrate zona-free hamster ova was positively but not significantly correlated with the sperm head ultrastructure, suggesting the two techniques may assess different aspects of sperm fertility.

Development of a zona-free hamster ova bioassay for goat sperm. Publishing Authors By Initials

T Berger,

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Development of a zona-free hamster ova bioassay for goat sperm. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 32

Page Numbers: 69-77

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Jul

YEAR: 1989

Development of a zona-free hamster ova bioassay for goat sperm. Information

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LANGUAGE: eng

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AFFILIATION: Department of Animal Science University of California, Davis Davis, CA 95616 USA.

Country: United States

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MEDLINETA: Theriogenology

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