Development of a well-defined medium for the in vitro maturation of immature bovine cumulus-oocyte complexes.

Development of a well-defined medium for the in vitro maturation of immature bovine cumulus-oocyte complexes. Research Abstract Details 

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Development of a well-defined medium for the in vitro maturation of immature bovine cumulus-oocyte complexes. Abstract Text:

PURPOSE: Our purpose was to develop a well-defined medium for the in vitro maturation (IVM) of immature bovine cumulus-oocyte complexes (COC). METHODS: The COC were cultured in the presence of three protein supplementations: fetal bovine serum (FBS), bovine serum albumin, and Synthetic Serum Substitute. The embryos obtained after in vitro fertilization of IVM oocytes were cocultured with Vero cells and their development to the morula and blastocyst stages was studied. RESULTS: When FBS was absent from the IVM medium, a significantly lower fertilization rate was observed, followed by a decrease in the percentage of embryos reaching the blastocyst stage. When FBS was replaced by a defined protein supplementation, the best results were obtained with Synthetic Serum Substitute. CONCLUSIONS: Adequate protein supplementation of the IVM medium optimizes the fertilization rate and the development of bovine IVM oocytes. The implication of these results in the human field is discussed.

Development of a well-defined medium for the in vitro maturation of immature bovine cumulus-oocyte complexes. Publishing Authors By Initials

For similar cells: cells, cultured: cell line: vero cells research abstracts see: cells: cells, cultured: cell line: vero cells research

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Development of a well-defined medium for the in vitro maturation of immature bovine cumulus-oocyte complexes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of assisted reproduction and genetics

VOLUME: 18

Page Numbers: 97-105

Journal Abbreviation: J. Assist. Reprod. Genet.

ISSN: 1058-0468

DAY: 17

MONTH: Feb

YEAR: 2001

Development of a well-defined medium for the in vitro maturation of immature bovine cumulus-oocyte complexes. Information

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LANGUAGE: eng

NlmUniqueID: 9206495

Development of a well-defined medium for the in vitro maturation of immature bovine cumulus-oocyte complexes. Keywords Mesh Terms:

KEYWORDS: Vero Cells

MESH TERMS: physiology

Chemical & Substance for Abstract: Development of a well-defined medium for the in vitro maturation of immature bovine cumulus-oocyte complexes. Information

Substance Name: Serum Albumin, Bovine

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Grant and Affiliation Information for Development of a well-defined medium for the in vitro maturation of immature bovine cumulus-oocyte complexes.

AFFILIATION: Unit of Reproductive Medicine, Department of Obstetrics and Gynecology, CHUV, CH-1011 Lausanne, Switzerland. alain.chanson@chuv.hospvd.ch

Country: United States

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MEDLINETA: J Assist Reprod Genet

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