Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity.

Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Abstract Text:

Yayoi Shimada,Takeshi Goto,Seiji Kawamoto,Takashi Kiso,Akiko Katayama,Yasushi Yamanaka,Tsunehiro Aki,Kuei-Chen Chiang,Toshiaki Nakano,Shigeru Goto,Chao-Long Chen,Naoya Ohmori,Kazuhisa Ono,Shuji Sato,Yayoi Shimada,Takeshi Goto,Seiji Kawamoto,Takashi Kiso,Akiko Katayama,Yasushi Yamanaka,Tsunehiro Aki,Kuei-Chen Chiang,Toshiaki Nakano,Shigeru Goto,Chao-Long Chen,Naoya Ohmori,Kazuhisa Ono,Shuji Sato,

In organ transplantation, the development of a novel immunosuppressant free of the need for permanent administration and any serious side effects has eagerly been awaited. We have previously reported that an anti-histone H1 polyclonal antibody has immunosuppressant activity. Here we prepared an anti-histone H1 monoclonal antibody as an analytical tool to elucidate its mechanism of immunosuppression. The isotype of this monoclonal antibody was immunoglobulin M. A monoclonal antibody prepared for administration to organ transplantation model animals should not contain any allogenic proteins and should have high purity. Therefore, we conducted a two-step chromatography procedure, consisting of strong anion-exchange chromatography and gel filtration chromatography, to purify an anti-histone H1 monoclonal immunoglobulin M antibody from the serum-free culture supernatant of hybridomas. Consequently, we successfully purified the monoclonal antibody at 96%, a purification rate at which its administration to organ transplantation model animals is possible. Copyright (c) 2007 John Wiley & Sons, Ltd.

Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Publishing Authors By Initials

Y Shimada,T Goto,S Kawamoto,T Kiso,A Katayama,Y Yamanaka,T Aki,KC Chiang,T Nakano,S Goto,CL Chen,N Ohmori,K Ono,S Sato,Y Shimada,T Goto,S Kawamoto,T Kiso,A Katayama,Y Yamanaka,T Aki,KC Chiang,T Nakano,S Goto,CL Chen,N Ohmori,K Ono,S Sato,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biomedical chromatography : BMC

VOLUME: 22

Page Numbers: 13-9

Journal Abbreviation: Biomed. Chromatogr.

ISSN: 0269-3879

DAY: 20

MONTH: Jan

YEAR: 2008

Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8610241

Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity.

AFFILIATION: Kazusa Institute for Drug Discovery, Josai International University, 2-1-6 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biomed Chromatogr

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity Related Publications

• Age related changes in the distribution of laryngeal glands in the human adult larynx.
• Anti-eosinophil peroxidase antibodies detected in patients with primary biliary cirrhosis.
• Assessment of bull fertility using a mucus penetration test and a human chorionic gonadotrophin stimulation test.
• Colonic mucosal lesions associated with long-term administration of non-steroidal anti-inflammatory drugs.
• Decreased expression levels of CD22 and L-selectin on peripheral blood B lymphocytes from patients with bullous pemphigoid.
• Differences in acetazolamide vasoreactivity in patients with acute and chronic occlusion of the internal carotid artery.
• Effect of low-molecular-weight heparin on the commitment of bone marrow cells to liver sinusoidal endothelial cells in CCl(4)-induced liver injury.
• Effects of gonadotropin releasing hormone and prostaglandin F(2)alpha on the reproductive performance in postpartum cows.
• Exacerbation of dietary steatohepatitis and fibrosis in obese, diabetic KK-A(y) mice.
• Expression of MUC1 and MUC2 mucin antigens in intrahepatic bile duct tumors: its relationship with a new morphological classification of cholangiocarcinoma.
• Expression of MUC1 and MUC2 mucins in gastric carcinomas: its relationship with the prognosis of the patients.
• Expression of mucin antigens in human cancers and its relationship with malignancy potential.
• Gene expression of gastric type mucin (MUC5AC) in pancreatic tumors: its relationship with the biological behavior of the tumor.
• Hepatic steatosis is a predictor of poor response to interferon alpha-2b and ribavirin combination therapy in Japanese patients with chronic hepatitis C.
• Immunohistochemical study of mucin carbohydrates and core proteins in human pancreatic tumors.
• Infective Endocarditis Associated with Sjogren's Syndrome.
• Isokinetic external rotation strength of shoulder : correlation with age and muscle size.
• MUC-1 mucin expression in invasive areas of intraductal papillary mucinous tumors of the pancreas.
• MUC2 gene expression is found in noninvasive tumors but not in invasive tumors of the pancreas and liver: its close relationship with prognosis of the patients.
• Mechanism by which Bombyx mori hemocytes recognize microorganisms: direct and indirect recognition systems for PAMPs.
• Modeling of daily operation in proton radiotherapy by Monte Carlo method.
• Mucin antigen expression in gastric carcinomas of young and old adults.
• Prostaglandin E(2) levels in uterine tissues and its relationship with uterine and luteal progesterone during the estrous cycle in dairy cows.
• Reconstruction of scalp and cranium defect utilizing latissimus dorsi musculocutaneous and serratus anterior muscle free flaps with interpositional anastomosis of T-shaped flap artery: case report.
• Second messenger systems mediating sex pheromone and amino Acid sensitivity in goldfish olfactory receptor neurons.
• Study on the appearance of the cavity in the corpus luteum of cows by using ultrasonic scanning.
• The expression of several types of mucin is related to the biological behavior of pancreatic neoplasms.
• The role of leptin in progression of non-alcoholic fatty liver disease.
• Voglibose potentiates the hepatotoxicity of carbon tetrachloride and acetaminophen by inducing CYP2E1 in rats.
• [Development and operation of cultured cell experiment with TR-IA sounding rocket]