Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola.

Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola. Abstract Text:

Inge N A Van Bogaert,Sofie L De Maeseneire,Dirk Develter,Wim Soetaert,Erick J Vandamme,Inge N A Van Bogaert,Sofie L De Maeseneire,Dirk Develter,Wim Soetaert,Erick J Vandamme,

Sophorolipids are surface-active compounds synthesized by the non-pathogenic yeast Candida bombicola. Over recent decades much effort has been spent to optimize culture conditions in order to improve the yield and production process. As far as we know, however, hardly any attention has been given to the genetics of the producing yeast strain itself and there are no published results available on the genetic engineering of C. bombicola. Nevertheless, this can be a useful tool for the study of the sophorolipid synthesis pathway and open up perspectives for improved production. A first step is the development of a suitable transformation and selection method. This article describes the creation and selection of an uracil auxotrophic C. bombicola mutant, which can be transformed back to prototrophy with the species' own orotidine 5'-phosphate decarboxylase or URA3 gene. Successful transformation was confirmed by a PCR-based method discriminating between the wild-type and mutated URA3 gene. Copyright (c) 2008 John Wiley & Sons, Ltd.

Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola. Publishing Authors By Initials

IN Van Bogaert,SL De Maeseneire,D Develter,W Soetaert,EJ Vandamme,IN Van Bogaert,SL De Maeseneire,D Develter,W Soetaert,EJ Vandamme,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Yeast (Chichester, England)

VOLUME: 25

Page Numbers: 273-8

Journal Abbreviation:

ISSN: 1097-0061

DAY: 3

MONTH: Apr

YEAR: 2008

Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8607637

Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola.

AFFILIATION: Laboratory of Industrial Microbiology and Biocatalysis, Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Yeast

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola Related Publications

• 'No willpower required'. The slimming industry and the right to sell dreams.
• A Computer-aided noninterfering on-line technique for monitoring oxygen-transfer characteristics during fermentation processes.
• Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex.
• Burkholderia sabiae sp. nov., isolated from root nodules of Mimosa caesalpiniifolia.
• Burkholderia sartisoli sp. nov., isolated from a polycyclic aromatic hydrocarbon-contaminated soil.
• Cerulenin inhibits de novo sophorolipid synthesis of Candida bombicola.
• Cloning and characterisation of the glyceraldehyde 3-phosphate dehydrogenase gene of Candida bombicola and use of its promoter.
• Cloning and characterization of the NADPH cytochrome P450 reductase gene (CPR) from Candida bombicola.
• Cloning and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene and the use of its promoter for expression in Myrothecium gramineum, a novel expression host.
• Collimonas arenae sp. nov. and Collimonas pratensis sp. nov., isolated from (semi-)natural grassland soils.
• Comparative performance of different PCR assays for the identification of Campylobacter jejuni and Campylobacter coli.
• Comparison of different strategies to reduce acetate formation in Escherichia coli.
• Comparison of protein quantification and extraction methods suitable for E. coli cultures.
• Development of a selection system for the detection of L-ribose isomerase expressing mutants of Escherichia coli.
• Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola.
• Devriesea agamarum gen. nov., sp. nov., a novel actinobacterium associated with dermatitis and septicaemia in agamid lizards.
• Estimation of an in vivo fitness landscape experienced by HIV-1 under drug selective pressure useful for prediction of drug resistance evolution during treatment.
• Leuconostoc holzapfelii sp. nov., isolated from Ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of Leuconostoc species.
• Matrix-assisted laser desorption ionisation-time-of of-flight mass spectrometry of intact cells allows rapid identification of Burkholderia cepacia complex.
• Minimizing acetate formation in E. coli fermentations.
• Modelled in vivo HIV fitness under drug selective pressure and estimated genetic barrier towards resistance are predictive for virological response.
• Phenotypic and molecular assessment of antimicrobial resistance in Lactobacillus paracasei strains of food origin.
• Rapid isolation of fungal genomic DNA suitable for long distance PCR.
• Renal replacement therapy in acute renal failure in children.
• The adaptation of the facade sound insulation requirements in the Belgian standard.
• The epidemic history of hepatitis C among injecting drug users in Flanders, Belgium.
• The genus Gluconobacter oxydans: comprehensive overview of biochemistry and biotechnological applications.
• Understanding the Changing Prevalence of K65R (Hot News).