Development of a storage-compatible microtiter plate-based technique for lymphocyte proliferation.

Development of a storage-compatible microtiter plate-based technique for lymphocyte proliferation. Research Abstract Details 

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Development of a storage-compatible microtiter plate-based technique for lymphocyte proliferation. Abstract Text:

R P Kerr,S Kim,S Ansar Ahmed,Steven D Holladay,R M Gogal,

The lymphocyte proliferation assay (LPA) is an important tier 1 test to assess non-specific lymphocyte function, in vitro. However, this assay requires fresh preparation, is time consuming, and labor intensive. Developing a plate coating technique for lymphocyte proliferation that is both stable and storage compatible would be useful to the basic and clinical researcher. In this study, we compared the effects of different mitogen plate coating techniques on lymphocyte proliferation to freshly prepared plates. The results show that plates prepared with complete media and stored at -40 degrees C up to 10 days corresponded well to control plates.

Development of a storage-compatible microtiter plate-based technique for lymphocyte proliferation. Publishing Authors By Initials

RP Kerr,S Kim,SA Ahmed,SD Holladay,RM Gogal,

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Development of a storage-compatible microtiter plate-based technique for lymphocyte proliferation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of immunoassay & immunochemistry

VOLUME: 29

Page Numbers: 128-42

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ISSN: 1532-4230

DAY: 24

MONTH: 03

YEAR: 2008

Development of a storage-compatible microtiter plate-based technique for lymphocyte proliferation. Information

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LANGUAGE: eng

NlmUniqueID: 100963688

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Grant and Affiliation Information for Development of a storage-compatible microtiter plate-based technique for lymphocyte proliferation.

AFFILIATION: The Center for Molecular Medicine and Infectious Diseases, Virginia Tech, Blacksburg, VA 24060-0342, USA.

Country: United States

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MEDLINETA: J Immunoassay Immunochem

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