Development of a monitoring vector for Leuconostoc mesenteroides using the green fluorescent protein gene.

Development of a monitoring vector for Leuconostoc mesenteroides using the green fluorescent protein gene. Research Abstract Details 

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Development of a monitoring vector for Leuconostoc mesenteroides using the green fluorescent protein gene. Abstract Text:

Kwan Hoon Lee,Woo Jung Park,Joo Yun Kim,Han Geun Kim,Jung Min Lee,Jeong Hwan Kim,Jeong Woo Park,Jong Hoon Lee,Sung Kyun Chung,Dae Kyun Chung,Kwan Hoon Lee,Woo Jung Park,Joo Yun Kim,Han Geun Kim,Jung Min Lee,Jeong Hwan Kim,Jeong Woo Park,Jong Hoon Lee,Sung Kyun Chung,Dae Kyun Chung,

The vector pCW5 with plasmid pC7, originally isolated in Lactobacillus paraplantarum C7 derived from kimchi, was constructed using a p32 strong promoter, the pC7 replicon, and green fluorescent protein (GFP) as the reporter. The constructed vector was transformed into E. coli and Leuconostoc mesenteroides, and GFP expression detected using a Western blot analysis. GFP fluorescence was recognized in E. coli and Leuconostoc mesenteroides using a confocal microscope. In addition, GFP fluorescence was also clearly detected in several industrially important lactic acid bacteria (LAB), including Lactobacillus bulgaricus, Lactobacillus paraplantarum, and Lactobacillus plantarum. Thus, pCW5 was shown to be effective for Leuconostoc mesenteroides when using GFP as the reporter, and it can also be used as a broad-host-range vector for other lactic acid bacteria.

Development of a monitoring vector for Leuconostoc mesenteroides using the green fluorescent protein gene. Publishing Authors By Initials

KH Lee,WJ Park,JY Kim,HG Kim,JM Lee,JH Kim,JW Park,JH Lee,SK Chung,DK Chung,KH Lee,WJ Park,JY Kim,HG Kim,JM Lee,JH Kim,JW Park,JH Lee,SK Chung,DK Chung,

For similar genetic processes: recombination, genetic: transformation, genetic research abstracts see: genetic processes: recombination, genetic: transformation, genetic research

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Development of a monitoring vector for Leuconostoc mesenteroides using the green fluorescent protein gene. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of microbiology and biotechnology

VOLUME: 17

Page Numbers: 1213-6

Journal Abbreviation: J. Microbiol. Biotechnol.

ISSN: 1017-7825

DAY: 24

MONTH: Jul

YEAR: 2007

Development of a monitoring vector for Leuconostoc mesenteroides using the green fluorescent protein gene. Information

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LANGUAGE: eng

NlmUniqueID: 9431852

Development of a monitoring vector for Leuconostoc mesenteroides using the green fluorescent protein gene. Keywords Mesh Terms:

KEYWORDS: Transformation, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: Development of a monitoring vector for Leuconostoc mesenteroides using the green fluorescent protein gene. Information

Substance Name: Luciferases

Registry Number: EC 1.13.12.-

Grant and Affiliation Information for Development of a monitoring vector for Leuconostoc mesenteroides using the green fluorescent protein gene.

AFFILIATION: Graduate School of Biotechnology and Biomaterial Technology and Business Center, Institute of Life Science and Resources, Kyung Hee University, Suwon 449-701, Korea.

Country: Korea (South)

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MEDLINETA: J Microbiol Biotechnol

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