Development of a molecular assay to detect predation on Anopheles gambiae complex larval stages.

Development of a molecular assay to detect predation on Anopheles gambiae complex larval stages. Research Abstract Details 

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Development of a molecular assay to detect predation on Anopheles gambiae complex larval stages. Abstract Text:

Erika Schielke,Carlo Costantini,Gianmaria Carchini, Sagnon,Jeffrey Powell,Adalgisa Caccone,

We developed a molecular assay to detect predation on Anopheles gambiae sensu lato (s.l.) mosquitoes. This intergenic spacer ribosomal DNA polymerase chain reaction assay and restriction enzyme analysis uses An. gambiae-specific primers to detect mosquito DNA in the DNA extracts from whole invertebrate predators, which enables identification of species (An. gambiae s.s. versus An. arabiensis) and molecular forms (M versus S in An. gambiae s.s.). We show that An. gambiae s.l. DNA can be detected after ingestion by members of the families Lestidae (order Odonata) after four hours, Libellulidae (order Odonata) after six hours, and Notonectidae (order Hemiptera) after 24 hours. This method is an improvement over previously published methods because of ease of execution and increased time of detection after ingestion.

Development of a molecular assay to detect predation on Anopheles gambiae complex larval stages. Publishing Authors By Initials

E Schielke,C Costantini,G Carchini,N Sagnon,J Powell,A Caccone,

For similar behavior and behavior mechanisms: behavior: behavior, animal: appetitive behavior: predatory behavior research abstracts see: behavior and behavior mechanisms: behavior: behavior, animal: appetitive behavior: predatory behavior research

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Development of a molecular assay to detect predation on Anopheles gambiae complex larval stages. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The American journal of tropical medicine and hygi

VOLUME: 77

Page Numbers: 464-6

Journal Abbreviation: Am. J. Trop. Med. Hyg.

ISSN: 0002-9637

DAY: 3

MONTH: Sep

YEAR: 2007

Development of a molecular assay to detect predation on Anopheles gambiae complex larval stages. Information

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LANGUAGE: eng

NlmUniqueID: 370507

Development of a molecular assay to detect predation on Anopheles gambiae complex larval stages. Keywords Mesh Terms:

KEYWORDS: Predatory Behavior

MESH TERMS: physiology

Chemical & Substance for Abstract: Development of a molecular assay to detect predation on Anopheles gambiae complex larval stages. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Development of a molecular assay to detect predation on Anopheles gambiae complex larval stages.

AFFILIATION: Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI46018

ACRONYM: AI

MEDLINETA: Am J Trop Med Hyg

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