Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus.

Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus. Research Abstract Details 

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Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus. Abstract Text:

Bipin Raj Bista,Chandra Ishwad,Robert M Wadowsky,Pradip Manna,Parmjeet Singh Randhawa,Gaurav Gupta,Meena Adhikari,Rakhi Tyagi,Gina Gasper,Abhay Vats,

Loop-mediated isothermal amplification (LAMP) is a novel method for rapid amplification of DNA. Its advantages include rapidity and minimal equipment requirement. The LAMP assay was developed for BK virus (BKV), which is a leading cause of morbidity in renal transplant recipients. The characteristics of the assay, including its specificity and sensitivity, were evaluated. BKV LAMP was performed using various incubation times with a variety of specimens, including unprocessed urine and plasma samples. A ladder pattern on gel electrophoresis, typical of successful LAMP reactions, was observed specifically only for BKV and not for other viruses. The sensitivity of the assay with 1 h of incubation was 100 copies/tube of a cloned BKV fragment. Additionally, a positive reaction was visually ascertained by a simple color reaction using SYBR green dye. BKV LAMP was also successful for urine and plasma specimens without the need for DNA extraction. Due to its simplicity and specificity, the LAMP assay can potentially be developed for "point of care" screening of BKV.

Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus. Publishing Authors By Initials

BR Bista,C Ishwad,RM Wadowsky,P Manna,PS Randhawa,G Gupta,M Adhikari,R Tyagi,G Gasper,A Vats,

For similar virus diseases: tumor virus infections research abstracts see: virus diseases: tumor virus infections research

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Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of clinical microbiology

VOLUME: 45

Page Numbers: 1581-7

Journal Abbreviation:

ISSN: 0095-1137

DAY: 21

MONTH: 02

YEAR: 2007

Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus. Information

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LANGUAGE: eng

NlmUniqueID: 7505564

Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus. Keywords Mesh Terms:

KEYWORDS: Tumor Virus Infections

MESH TERMS: virology

Chemical & Substance for Abstract: Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus. Information

Substance Name: DNA, Viral

Registry Number: 0

Grant and Affiliation Information for Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus.

AFFILIATION: Department of Pediatrics, Children's Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, PA 15213, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI060602

ACRONYM: AI

MEDLINETA: J Clin Microbiol

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