Development of a Lepto-IgM EIACR test to diagnose leptospirosis disease in Costa Rican patient samples.

Development of a Lepto-IgM EIACR test to diagnose leptospirosis disease in Costa Rican patient samples. Research Abstract Details 

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Development of a Lepto-IgM EIACR test to diagnose leptospirosis disease in Costa Rican patient samples. Abstract Text:

de los A Valverde,Bernal ,Lizeth Taylor,Kirsten Visona,

Leptospirosis is an endemic disease throughout Costa Rica, which could be misdiagnosed because manifestations of this febrile disease may vary from mild flu-like symptoms to severe illness involving vital organs such as liver and lungs. Therefore an early specific diagnosis is important to ensure a favorable clinical outcome. The purpose of this study was to develop a Leptospira sp. anti-IgM EIA (Lepto-IgM EIACR) test and to compare it using Lepto-Dipstick IgM (Lepto-DS IgM) and PanBio-EIA IgM with the Microscopy Agglutination test (MAT) as a reference assay. Sera from 736 healthy blood donors were used as negative controls to calculate specificity (97.1%), Confidence Interval 95 (CI (96-98). Cross reactivity was evaluated in 268 patient samples with 6 different diseases. Dengue and measles had the highest cross reactivity (16%) while rubella showed the lowest (3%). To determine the sensitivity of the Lepto- IgM EIACR, 33 samples positive by MAT of 96 paired samples from patients with symptoms related to leptospirosis infection were tested. Lepto-IgM EIACR reached a sensitivity of 90.9% (CI 81-100), while Lepto-DS IgM was 48.5% (CI (31-66). The most frequent serovars detected by MAT in these paired samples were Hebdomadis 14.7%, Hardjo 11.8%, Pomona 8.8% and Icterohaemorrhagiae 5.9%. Furthermore 59 febrile patient samples were tested initially with PanBio-EIA IgM, 21 samples (35%) were positive. When these samples were re-tested by Lepto-IgM EIACR and Lepto-DS IgM, 80.9% and 33% were positive, respectively. The results of the evaluation indicate that Lepto-IgM EIACR test could be a good alternative to detect acute leptospirosis in Costa Rica.

Development of a Lepto-IgM EIACR test to diagnose leptospirosis disease in Costa Rican patient samples. Publishing Authors By Initials

M de los A Valverde,B ,L Taylor,K Visona,

For similar virus diseases research abstracts see: virus diseases research

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Development of a Lepto-IgM EIACR test to diagnose leptospirosis disease in Costa Rican patient samples. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Investigación clínica

VOLUME: 48

Page Numbers: 295-304

Journal Abbreviation:

ISSN: 0535-5133

DAY: 3

MONTH: Sep

YEAR: 2007

Development of a Lepto-IgM EIACR test to diagnose leptospirosis disease in Costa Rican patient samples. Information

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LANGUAGE: eng

NlmUniqueID: 421531

Development of a Lepto-IgM EIACR test to diagnose leptospirosis disease in Costa Rican patient samples. Keywords Mesh Terms:

KEYWORDS: Virus Diseases

MESH TERMS: diagnosis

Chemical & Substance for Abstract: Development of a Lepto-IgM EIACR test to diagnose leptospirosis disease in Costa Rican patient samples. Information

Substance Name: Reagent Kits, Diagnostic

Registry Number: 0

Grant and Affiliation Information for Development of a Lepto-IgM EIACR test to diagnose leptospirosis disease in Costa Rican patient samples.

AFFILIATION: Instituto Costarricense de Investigación y Enseñianza en Nutrición y Salud, INCIENSA, Tres Ríos, San José, Costa Rica. mvalverde@inciensa.sa.cr

Country: Venezuela

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MEDLINETA: Invest Clin

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