Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood.

Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood. Research Abstract Details 

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Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood. Abstract Text:

Hua Yu,Jason M K Ho,Kelvin K W Kan,Bobby W H Cheng,Wen-Ming Li,Li Zhang,Ge Lin,Yuan-Ping Pang,Zhe-Ming Gu,Kelvin Chan,Yi-Tao Wang,Yi-Fan Han,

An analytical method using on-line high performance liquid chromatography-tandem mass spectrometry with electrospray ionization was developed and applied for the quantification of bis(7)-tacrine (B7T) in rat blood. B7T and pimozide (internal standard, IS) were extracted in a single step from 100 microl of alkalized blood with ethyl acetate. Analytes were separated using an Extend C-18 column at 25 degrees C. The elution was achieved isocratically with a mobile phase composed of 0.05% aqueous formic acid and acetonitrile (60:40, v/v) at a flow rate of 0.35 ml/min. Quantification was achieved by monitoring the selected ions at m/z 247 for B7T and m/z 462-->m/z 328 for pimozide. Retention times were 1.45 and 2.23 min for B7T and IS, respectively. Calibration curves were linear in the range from 86.4 to 2160.0 ng/ml. The established method is rapid, selective and sensitive for the identification and quantification of B7T in biological samples. The assay is accurate (bias <10%) and reproducible (intra- and inter-day variation <10%), with detection and quantification limit of 3.6 and 42.3 ng/ml, respectively. Furthermore, it was successfully applied for the pharmacokinetic measurement of B7T in rat with a single intravenous administration at 0.3mg/kg.

Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood. Publishing Authors By Initials

H Yu,JM Ho,KK Kan,BW Cheng,WM Li,L Zhang,G Lin,YP Pang,ZM Gu,K Chan,YT Wang,YF Han,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of pharmaceutical and biomedical analysis

VOLUME: 44

Page Numbers: 1133-8

Journal Abbreviation:

ISSN: 0731-7085

DAY: 25

MONTH: 05

YEAR: 2007

Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood. Information

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LANGUAGE: eng

NlmUniqueID: 8309336

Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: methods

Chemical & Substance for Abstract: Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood. Information

Substance Name: Tacrine

Registry Number: 321-64-2

Grant and Affiliation Information for Development of a high performance liquid chromatography-tandem mass method for determination of bis(7)-tacrine, a promising anti-Alzheimer's dimer, in rat blood.

AFFILIATION: Department of Biochemistry, Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, SAR, PR China.

Country: England

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MEDLINETA: J Pharm Biomed Anal

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