Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken.

Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken. Abstract Text:

We describe a polymerase chain reaction (PCR)-based assay for the detection, identification and differentiation of pathogenic species of .Eimeria in poultry. The internal transcribed spacer 1 (ITS1) regions of ribosomal DNA (rDNA) from .Eimeria acervulina, E. brunetti, E. necatrix and .E. tenella were sequenced and regions of unique sequences identified. Four pairs of oligonucleotide primers, each designed to amplify the ITS1 region of a single .Eimeria species, were synthesised for use in the PCR assay. In tests on purified genomic DNA from all seven species of .Eimeria that infect the chicken, each of the four primer pairs amplified the ITS1 region from only their respective target species. The robustness of the approach was further demonstrated by the amplification of specific DNA fragments from tissues of experimentally infected animals and from oocysts recovered from field samples. We conclude that the ITS1 regions of .Eimeria species contain sufficient inter-specific sequence variation to enable the selection of primers that can be applied in PCR analyses to detect and differentiate between species. In future work they may provide excellent markers for epidemiological studies.

Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Avian pathology : journal of the W.V.P.A

VOLUME: 27

Page Numbers: 490-7

Journal Abbreviation: Avian Pathol.

ISSN: 0307-9457

DAY: 16

MONTH: Oct

YEAR: 1998

Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8210638

Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken.

AFFILIATION: Department of Parasitology, Swedish University of Agricultural Sciences & National Veterinary Institute, Uppsala, Sweden.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Avian Pathol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic Eimeria species of the chicken Related Publications

• A 55-year-old man with right-sided weakness.
• An overview of divalent cation and citrate handling by the kidney.
• Autopsy rates at the University Hospital of the West Indies, 1968-1997.
• Behavior problems in pediatric practice.
• Beyond fibrocystic disease. The evolving concept of pre-malignant breast disease.
• CURRENT THINKING IN NUTRITION OF DENTAL TISSUES.
• Debate about non-heart beating organ donation.
• Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic .Eimeria species of the chicken.
• Fibrolytic enzymes to increase the nutritive value of dairy feedstuffs.
• Matrix extracellular phosphoglycoprotein causes phosphaturia in rats by inhibiting tubular phosphate reabsorption.
• PEDIATRICIAN'S POINT OF VIEW.
• Revised formulas for diffraction effects with point and extended sources.
• Room-in for mothers and infants.
• Ruminal degradability and lysine bioavailability of soybean meals and effects on performance of dairy cows.
• Studies on the pathogenicity of chicken-maintained (virulent) and embryo-adapted (attenuated) strains of Eimeria Mivati.
• Survival in lung cancer.
• Testicular metastases from carcinoma of the prostate.
• The Safer Patients Initiative: the UK experience of attempting to improve safe clinical care.
• The control of hypertension by use of coconut water and mauby: two tropical food drinks.
• The development of collective moral leadership among parents through education organizing.
• The effect of vasopressin on renal blood flow and its distribution in the rat.
• The isolation of crystalline tennecetin and the comparison of this antibiotic with pimaricin.
• Time-of-flight photoelectron spectroscopy of gases using synchrotron radiation.