Development and validation of an assay for the quantitative determination of cladribine nucleotides in MDCKII cells and culture medium using weak anion-exchange liquid chromatography coupled with tandem mass spectrometry.

Development and validation of an assay for the quantitative determination of cladribine nucleotides in MDCKII cells and culture medium using weak anion-exchange liquid chromatography coupled with tandem mass spectrometry. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Development and validation of an assay for the quantitative determination of cladribine nucleotides in MDCKII cells and culture medium using weak anion-exchange liquid chromatography coupled with tandem mass spectrometry. Abstract Text:

Robert S Jansen,Hilde Rosing,Cocky J F de Wolf,Jos H Beijnen,Robert S Jansen,Hilde Rosing,Cocky J F de Wolf,Jos H Beijnen,

The development and validation of an assay for the quantitative analysis of cladribine mono-, di- and triphosphate (2-chloro, 2'-deoxyadenosine 5'-mono-, di- and triphosphate or 2CdAMP, 2CdADP and 2CdATP) in culture medium (Optimem) and cell lysate is described. Cladribine mono- and diphosphate reference compounds were obtained by thermal degradation of cladribine triphosphate. The reference compounds were characterized using ion-pairing reversed-phase high-performance liquid chromatography with ultraviolet detection. The bioanalytical assay for 2CdAMP, 2CdADP and 2CdATP is based on weak anion-exchange liquid chromatography coupled with tandem mass spectrometry in the positive ion mode (WAXLC/MS/MS). A fused-silica electrospray capillary was used instead of a stainless steel electrospray capillary to minimize adsorption of analytes and thus decrease variation in the analyte signals. Dynamic ranges of 1.11-27.7, 0.550-55.0 and 1.31-52.3 nM for 2CdAMP, 2CdADP and 2CdATP, respectively, were validated in culture medium and cell lysate. Optimem samples required stabilization with 30% methanol to prevent conversion of 2CdATP into 2CdAMP and 2CdADP. All intra- and interday accuracies and precisions were within +/-20%. The stability of the compounds was assessed under various analytically relevant conditions. The method was successfully used to investigate cladribine nucleotide transport in vitro in Madin-Darby canine kidney II (MDCKII) cells.

Development and validation of an assay for the quantitative determination of cladribine nucleotides in MDCKII cells and culture medium using weak anion-exchange liquid chromatography coupled with tandem mass spectrometry. Publishing Authors By Initials

RS Jansen,H Rosing,CJ de Wolf,JH Beijnen,RS Jansen,H Rosing,CJ de Wolf,JH Beijnen,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Development and validation of an assay for the quantitative determination of cladribine nucleotides in MDCKII cells and culture medium using weak anion-exchange liquid chromatography coupled with tandem mass spectrometry. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Rapid communications in mass spectrometry : RCM

VOLUME: 21

Page Numbers: 4049-59

Journal Abbreviation: Rapid Commun. Mass Spectrom.

ISSN: 0951-4198

DAY: 6

MONTH: 12

YEAR: 2007

Development and validation of an assay for the quantitative determination of cladribine nucleotides in MDCKII cells and culture medium using weak anion-exchange liquid chromatography coupled with tandem mass spectrometry. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8802365

Development and validation of an assay for the quantitative determination of cladribine nucleotides in MDCKII cells and culture medium using weak anion-exchange liquid chromatography coupled with tandem mass spectrometry. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Development and validation of an assay for the quantitative determination of cladribine nucleotides in MDCKII cells and culture medium using weak anion-exchange liquid chromatography coupled with tandem mass spectrometry. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Development and validation of an assay for the quantitative determination of cladribine nucleotides in MDCKII cells and culture medium using weak anion-exchange liquid chromatography coupled with tandem mass spectrometry.

AFFILIATION: Department of Pharmacy & Pharmacology, Slotervaart Hospital/The Netherlands Cancer Institute, Louwesweg 6, Amsterdam, The Netherlands. robert.jansen@slz.nl

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Rapid Commun Mass Spectrom

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Development and validation of an assay for the quantitative determination of cladribine nucleotides in MDCKII cells and culture medium using weak anion-exchange liquid chromatography coupled with tandem mass spectrometry Related Publications

• A sensitive assay for the quantitative analysis of vinorelbine in mouse and human EDTA plasma by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry.
• Amyloid Beta protein and tau in cerebrospinal fluid and plasma as biomarkers for dementia: a review of recent literature.
• Amyloid beta protein and tau in cerebrospinal fluid and plasma as biomarkers for dementia: a review of recent literature.
• Carboplatin dosage formulae can generate inaccurate predictions of Carboplatin exposure in carboplatin/paclitaxel combination regimens.
• Comparing the old and new generation SELDI-TOF MS: implications for serum protein profiling.
• Development and validation of an assay for the quantitative determination of cladribine nucleotides in MDCKII cells and culture medium using weak anion-exchange liquid chromatography coupled with tandem mass spectrometry.
• Didanosine, a new antiretroviral drug. A review.
• Dideoxynucleoside therapy for HIV infection.
• Drug Interaction of Fluvoxamine and Fluoxetine with Nevirapine in HIV-1-Infected Individuals.
• Drug-drug interactions in a geriatric outpatient cohort: prevalence and relevance.
• Enhanced resolution triple-quadrupole mass spectrometry for ultra-sensitive and quantitative analysis of the investigational anticancer agent EO9 (apaziquone) and its metabolite EO5a in human and dog plasma to support (pre)-clinical studies of EOquin(R) g
• Evaluation of clinical pharmacist interventions on drug interactions in outpatient pharmaceutical HIV-care.
• Home-based chemotherapy confronts pharmacists with stability and compatibility problems.
• Liquid chromatography-tandem mass spectrometric assay for diclofenac and three primary metabolites in mouse plasma.
• Liquid chromatography-tandem mass spectrometric assays for salinomycin in mouse plasma, liver, brain and small intestinal contents and in OptiMEM cell culture medium.
• Liquid chromatography/tandem mass spectrometric method for the quantification of eight proteolytic fragments of ITIH(4) with biomarker potential in human plasma and serum.
• New cytotoxic drugs and targets in oncology.
• Pharmacokinetics and pharmacokinetic variability of heroin and its metabolites: review of the literature.
• Platinum antitumour agents: a review of (bio)analysis.
• Prescription error resulting in valproic acid intoxication.
• Quantitative and selective assay of 5-methylindirubine, an inhibitor of cyclin-dependent kinases, in murine plasma using coupled liquid chromatography and electrospray tandem mass spectrometry.
• Quantitative aspects of the analysis of the monoclonal antibody trastuzumab using high-performance liquid chromatography coupled with electrospray mass spectrometry.
• Quantitative bioanalysis of peptides by liquid chromatography coupled to (tandem) mass spectrometry.
• Serum Amyloid Beta Peptides in Patients with Dementia and Age-Matched Non-Demented Controls as Detected by Surface-Enhanced Laser Desorption Ionisation-Time of Flight Mass Spectrometry (SELDI-TOF MS).
• The risks of handling cytotoxic drugs. I. Methods of testing exposure.
• The risks of handling cytotoxic drugs. II. Recommendations for working with cytotoxic drugs.