Development and validation of a liquid chromatography/tandem mass spectrometry assay for the quantification of methyl protodioscin in rat plasma: application to a pharmacokinetic study.

Development and validation of a liquid chromatography/tandem mass spectrometry assay for the quantification of methyl protodioscin in rat plasma: application to a pharmacokinetic study. Research Abstract Details 

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Development and validation of a liquid chromatography/tandem mass spectrometry assay for the quantification of methyl protodioscin in rat plasma: application to a pharmacokinetic study. Abstract Text:

Xiuzhen Cao,Zhihong Yao,Haifeng Chen,Yi Dai,Pinghua Sun,Wencai Ye,Xinsheng Yao,

A high performance liquid chromatography/tandem mass spectrometry assay was first developed and validated for the quantification of methyl protodioscin (MPD), a natural furostanol saponin with distinct antitumor activity, in rat plasma with 17alpha-ethinylestradiol as internal standard (IS). Methanol-mediated protein precipitation was employed for plasma sample pretreatment. The separation was achieved on a C(18) column (150 x 4.6 mm, i.d., 5 microm) by isocratic elution with methanol-water (72:28, v/v) as mobile phase at a flow rate of 1.0 mL/min. Ion acquisition was performed in selective reaction monitoring positive mode by monitoring the transition of m/z 1085.7 --> 1053.7 for MPD, and in selective ion monitoring negative mode by monitoring the deprotonated ion m/z 295.5 for IS. The assay was linear over the concentration range of 2.024-270.0 microg/mL with 2.024 microg/mL as the lower limit of quantification. It was specific, accurate, precise and reproducible with intra- and inter-run RSD <8.3% and RE between -11.5 and 12.8%. The assay was successfully applied to a preclinical pharmacokinetic study after an intravenous dose of 40 mg/kg MPD to rats. Copyright (c) 2007 John Wiley & Sons, Ltd.

Development and validation of a liquid chromatography/tandem mass spectrometry assay for the quantification of methyl protodioscin in rat plasma: application to a pharmacokinetic study. Publishing Authors By Initials

X Cao,Z Yao,H Chen,Y Dai,P Sun,W Ye,X Yao,

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Development and validation of a liquid chromatography/tandem mass spectrometry assay for the quantification of methyl protodioscin in rat plasma: application to a pharmacokinetic study. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biomedical chromatography : BMC

VOLUME: 22

Page Numbers: 408-13

Journal Abbreviation: Biomed. Chromatogr.

ISSN: 0269-3879

DAY: 24

MONTH: Apr

YEAR: 2008

Development and validation of a liquid chromatography/tandem mass spectrometry assay for the quantification of methyl protodioscin in rat plasma: application to a pharmacokinetic study. Information

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LANGUAGE: eng

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AFFILIATION: Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Wenhua-Road 103, Shenyang 110016, People's Republic of China.

Country: England

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MEDLINETA: Biomed Chromatogr

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