Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas).

Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas). Research Abstract Details 

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Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas). Abstract Text:

Patrick Larkin,Daniel L Villeneuve,Iris Knoebl,Ann L Miracle,Barbara J Carter,Li Liu,Nancy D Denslow,Gerald T Ankley,

Gene microarrays provide the field of ecotoxicology new tools to identify mechanisms of action of chemicals and chemical mixtures. Herein we describe the development and application of a 2,000-gene oligonucleotide microarray for the fathead minnow Pimephales promelas, a species commonly used in ecological risk assessments in North America. The microarrays were developed from various cDNA and subtraction libraries that we constructed. Consistency and reproducibility of the microarrays were documented by examining multiple technical replicates. To test application of the fathead minnow microarrays, gene expression profiles of fish exposed to 17beta-estradiol, a well-characterized estrogen receptor (ER) agonist, were examined. For these experiments, adult male fathead minnows were exposed for 24 h to waterborne 17beta-estradiol (40 or 100 ng/L) in a flow-through system, and gene expression in liver samples was characterized. Seventy-one genes were identified as differentially regulated by estradiol exposure. Examination of the gene ontology designations of these genes revealed patterns consistent with estradiol's expected mechanisms of action and also provided novel insights as to molecular effects of the estrogen. Our studies indicate the feasibility and utility of microarrays as a basis for understanding biological responses to chemical exposure in a model ecotoxicology test species.

Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas). Publishing Authors By Initials

P Larkin,DL Villeneuve,I Knoebl,AL Miracle,BJ Carter,L Liu,ND Denslow,GT Ankley,

For similar investigative techniques: genetic techniques: nucleic acid amplification techniques: polymerase chain reaction research abstracts see: investigative techniques: genetic techniques: nucleic acid amplification techniques: polymerase chain reaction research

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Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas). Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Environmental toxicology and chemistry / SETAC

VOLUME: 26

Page Numbers: 1497-506

Journal Abbreviation: Environ. Toxicol. Chem.

ISSN: 0730-7268

DAY: 3

MONTH: Jul

YEAR: 2007

Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas). Information

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LANGUAGE: eng

NlmUniqueID: 8308958

Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas). Keywords Mesh Terms:

KEYWORDS: Polymerase Chain Reaction

MESH TERMS: genetics

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Grant and Affiliation Information for Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas).

AFFILIATION: EcoArray, 12085 Research Drive, Alachua, Florida 32615, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: 2R44ES013637-02

ACRONYM: ES

MEDLINETA: Environ Toxicol Chem

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