Development and preliminary application of a new assay for aldosterone stimulating factor.

Development and preliminary application of a new assay for aldosterone stimulating factor. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Development and preliminary application of a new assay for aldosterone stimulating factor. Abstract Text:

S Sen,F M Bumpus,S Oberfield,M I New,

A new assay method has been developed to measure aldosterone stimulating factor (ASF) quantitatively. This method utilizes a combination of affinity chromatography and high pressure liquid chromatography (HPLC). Antibodies raised against ASF, coupled to Affi-Gel 10, were used as the affinity column, and adsorbed ASF was eluted with 4 M urea. Quantitation was based on the external standard method of analysis using the area of the standards as controls, which were previously tested by HPLC for purity. The amount of ASF in the unknown samples was calculated on the basis of the area of the peak emerging at the retention time of ASF. A parallel bioassay using adrenal glomerulosa cells was also done to assess the biological activity. In eight normal volunteers, the urinary ASF was 146 +/- 4.6 ng/24 hr urine, whereas in plasma it was 70.5 +/- 6.5 ng/100 ml. The method is reproducible, specific for ASF, and showed less than 3% inter- or intraassay variability. In a preliminary study, when ASF was quantified from the 24-hour urine of two patients with adrenal hyperplasia, a significantly higher level of ASF was found (750 and 1020 ng/24 hr urine). These data suggest that ASF may be of pathological significance in certain hypertensive patients, especially in the hypertension associated with hyperaldosteronism.

Development and preliminary application of a new assay for aldosterone stimulating factor. Publishing Authors By Initials

S Sen,FM Bumpus,S Oberfield,MI New,

For similar proteins research abstracts see: proteins research

PUBMED ID PMID:

MEDLINE DATE: 1983 Mar-Apr

Development and preliminary application of a new assay for aldosterone stimulating factor. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Hypertension

VOLUME: 5

Page Numbers: I27-31

Journal Abbreviation: Hypertension

ISSN: 0194-911X

DAY: 19

MONTH: 11

YEAR: 2007

Development and preliminary application of a new assay for aldosterone stimulating factor. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7906255

Development and preliminary application of a new assay for aldosterone stimulating factor. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: immunology

Chemical & Substance for Abstract: Development and preliminary application of a new assay for aldosterone stimulating factor. Information

Substance Name: Aldosterone

Registry Number: 52-39-1

Grant and Affiliation Information for Development and preliminary application of a new assay for aldosterone stimulating factor.

AFFILIATION:

Country: UNITED STATES

AGENCY: United States NHLBI

GRANT: HL-6835

ACRONYM: HL

MEDLINETA: Hypertension

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Development and preliminary application of a new assay for aldosterone stimulating factor Related Publications

• Aldosterone-to-renin ratio as a marker for disease severity in 21-hydroxylase deficiency congenital adrenal hyperplasia.
• Amyloid tumors of the larynx, trachea or bronchi; a report of 15 cases.
• Cancer of the face; treatment and plastic repair.
• Congenital adrenal hyperplasia in adolescents: diagnosis and management.
• Development and preliminary application of a new assay for aldosterone stimulating factor.
• Effects of adrenal steroids on the bone metabolism of children with congenital adrenal hyperplasia.
• Ethnic-specific distribution of mutations in 716 patients with congenital adrenal hyperplasia owing to 21-hydroxylase deficiency.
• Exaggerated affect-modulated startle during unpleasant stimuli in borderline personality disorder.
• Excess-noise dependence on intracavity aperture shape.
• If you could read my mind. Implications of neurological evidence for twenty-first century criminal jurisprudence.
• Inactivation of the acid labile subunit gene resulting in insulin-like growth factor deficiency.
• Leiomyoma cutis (zosteriform).
• Low renin hypertension (LRH): shades of John Laragh.
• Optical carrier wave shocking: detection and dispersion.
• Paul F. J. New, MD, FACR.
• Percutaneous transluminal renal angioplasty in the treatment of renovascular hypertension in children.
• Pope Joan: a recognizable syndrome.
• Prenatal diagnosis and treatment of congenital adrenal hyperplasia owing to 21-hydroxylase deficiency.
• Prenatal diagnosis and treatment of congenital adrenal hyperplasia.
• Prenatal diagnosis and treatment of congenital adrenal hyperplasia.
• Report of Fertility in a Woman with a Predominantly 46,XY Karyotype in a Family with Multiple Disorders of Sexual Development.
• Report of fertility in woman with predominantly 46,XY karyotype in family with multiple disorders of sexual development: Review of Prismatic Case.
• Rhinoscleroma apparently cured with streptomycin.
• Sexual Orientation in Women with Classical or Non-classical Congenital Adrenal Hyperplasia as a Function of Degree of Prenatal Androgen Excess.
• Smooth surface micro finite element modelling of a cancellous bone analogue material.
• Somatomedin and growth hormone in psychosocial dwarfism.
• Steroid 11beta- hydroxylase deficiency congenital adrenal hyperplasia.
• The Gordon Wilson Lecture. Congenital adrenal hyperplasia.
• The Recalled Childhood Gender Questionnaire-Revised: a psychometric analysis in a sample of women with congenital adrenal hyperplasia.
• The effect of treatment of final height in classical congenital adrenal hyperplasia (CAH).