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Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon.

Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon. Research Abstract Details 

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  • Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon. Abstract Text:

    f j nogueraF J Noguera,j capelJ Capel,j i alvarezJ I Alvarez,r lozanoR Lozano,

    Monoecy is an important goal for melon breeding because of the agronomic advantages it provides to parental lines in that they do not require hand emasculation to develop monoecious F1 hybrids, the latter producing fruits of higher quality. Monoecious phenotype is conferred by the dominant allele of the andromonoecious (a) gene, whereas recessive homozygous plants are andromonoecious. A bulked segregant analysis (BSA) approach performed in a set of 38 double-haploid lines has allowed us to identify an AFLP marker linked to the a gene at 3.3 cM. Following cloning and sequencing of the AFLP fragment, specific PCR primers were designed and used in the amplification of a codominant SCAR marker. Using a backcrossed mapping population of 530 plants, the SCAR marker could be mapped near the a locus (5.5 cM). Size difference between the two allelic SCAR fragments is 42 bp and might be due to a deletion/insertion. The SCAR marker is closest to the a gene identified to date, and can be useful in breeding programs, using marker-assisted selection procedures to screen for sexual types in melon.

    Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon. Publishing Authors By Initials

    fj nogueraFJ Noguera,j capelJ Capel,ji alvarezJI Alvarez,r lozanoR Lozano,

    For similar genetic processes: sex determination (genetics) research abstracts see: genetic processes: sex determination (genetics) research

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    Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: TAG. Theoretical and applied genetics. Theoretisch

    VOLUME: 110

    Page Numbers: 714-20

    Journal Abbreviation: Theor. Appl. Genet.

    ISSN: 0040-5752

    DAY: 14

    MONTH: 01

    YEAR: 2005

    Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 145600

    Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon. Keywords Mesh Terms:

    KEYWORDS: Sex Determination (Genetics)

    MESH TERMS: genetics

    Chemical & Substance for Abstract: Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon. Information

    Substance Name: Genetic Markers

    Registry Number: 0

    Grant and Affiliation Information for Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon.

    AFFILIATION: Departamento de Biología Aplicada (Genética), Edificio CITE II-B, Universidad de Almería, La Cañada de San Urbano s/n, Almería, 04120, Spain.

    Country: Germany

    Germany Research PublicationGermany Research Publication

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    MEDLINETA: Theor Appl Genet

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