Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion.

Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Abstract Text:

Gi-Sun Im,Melissa Samuel,Liangxue Lai,Yanhong Hao,Randall S Prather,

This study investigated the effect of treatment with 6-dimethylaminopurine (6-DMAP) following fusion on in vitro development of porcine nuclear transfer (NT) embryos. Frozen thawed ear skin cells were transferred into the perivitelline space of enucleated oocytes. Reconstructed oocytes were fused and activated with electric pulse in 0.3 M mannitol supplemented with either 0.1 or 1.0 mM CaCl(2). In each calcium concentration, activated oocytes were divided into three groups. Two groups of them were exposed to either ionomycin (I + 6-DMAP or 6-DMAP alone. In experiment 2, fused NT embryos in 0.3 M mannitol containing 1.0 mM CaCl(2) were exposed to 6-DMAP either immediately or 20 min after fusion/activation. For 0.1 mM CaCl(2), oocytes activated with either I + 6-DMAP or 6-DMAP alone showed a higher (P < 0.05) developmental rate to the blastocyst stage than those activated with an electric pulse alone (26.7 and 22.5 vs. 12.5%). For 1.0 mM CaCl(2), oocytes activated with either I + 6-DMAP or 6-DMAP alone showed significantly higher (P < 0.05) developmental rate to the blastocyst stage (35.6 and 28.3 vs. 19.8%). Developmental rate to the blastocyst stage was (P < 0.05) increased in NT embryos activated with 6-DMAP 20 min after fusion. 6-DMAP made a higher and wider Ca(2+) transient compared to that induced by electric pulses (Fig. 3). The fluctuation lasted during the time that oocytes were cultured in 6-DMAP. Regardless of Ca(2+) concentration in fusion medium, activation with 6-DMAP following electric pulses supported more development of porcine NT embryos. Activation of NT embryos with 6-DMAP after fusion in the presence of 1.0 mM CaCl(2) could support better developmental rate to the blastocyst stage.

Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Publishing Authors By Initials

GS Im,M Samuel,L Lai,Y Hao,RS Prather,

For similar animals: chordata: vertebrates: mammals: artiodactyla: swine research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: swine research

PUBMED ID PMID:

MEDLINE DATE:

Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular reproduction and development

VOLUME: 74

Page Numbers: 1158-64

Journal Abbreviation: Mol. Reprod. Dev.

ISSN: 1040-452X

DAY: 29

MONTH: Sep

YEAR: 2007

Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8903333

Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Keywords Mesh Terms:

KEYWORDS: Swine

MESH TERMS: embryology

Chemical & Substance for Abstract: Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Information

Substance Name: N(6),N(6)-dimethyladenine

Registry Number: 938-55-6

Grant and Affiliation Information for Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion.

AFFILIATION: Division of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri 65211, USA.

Country: United States

AGENCY: United States NCRR

GRANT: R01 RR13438

ACRONYM: RR

MEDLINETA: Mol Reprod Dev

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion Related Publications

• A protein tyrosine phosphatase inhibitor, sodium orthovanadate, causes parthenogenetic activation of pig oocytes via an increase in protein tyrosine kinase activity.
• Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts.
• An anisotropy of human tactile sensitivity and its relation to the visual oblique effect.
• Carbohydrate tolerance and serum lipid responses to type of dietary carbohydrate and oral contraceptive use in young women.
• Cloned transgenic heart-healthy pork?
• Cloned transgenic swine via in vitro production and cryopreservation.
• Concentration and composition of free amino acids and osmolalities of porcine oviductal and uterine fluid and their effects on development of porcine IVF embryos.
• Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine.
• Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion.
• Diacylglycerol-enhanced electrical activation of porcine oocytes matured in vitro.
• Electromagnetic modeling of active silicon nanocrystal waveguides.
• End note:summary.
• Fiber-to-waveguide coupler based on the parabolic reflector.
• High renal ectopy; a case report.
• Hyaluronic acid fillers for the male patient.
• Investigations of the diurnal cycle and mixing state of oxalic acid in individual particles in Asian aerosol outflow.
• Juvéderm: a hyaluronic acid dermal filler.
• Multichannel wavelength division multiplexing with photonic crystals.
• Nuclear remodeling and nuclear reprogramming for making transgenic pigs by nuclear transfer.
• Osteopontin improves in vitro development of porcine embryos and decreases apoptosis.
• Photoresist characterization and linearization procedure for the gray-scale fabrication of diffractive optical elements.
• Plasma fructose, uric acid, and inorganic phosphorus responses of hyperinsulinemic men fed fructose.
• Pluristem Therapeutics, Inc.
• Pneumonephrosis; a complication of necrotizing pyelonephritis.
• Real-time, single-particle measurements of oligomers in aged ambient aerosol particles.
• Surgical treatment of serous cyst of the kidney.
• Targeted genetic modification: xenotransplantation and beyond.
• The CB2 cannabinoid agonist AM-1241 prolongs survival in a transgenic mouse model of amyotrophic lateral sclerosis when initiated at symptom onset.
• Thin film solar cell design based on photonic crystal and diffractive grating structures.
• Vector-based plane-wave spectrum method for the propagation of cylindrical electromagnetic fields.