Deuterium nuclear magnetic resonance spectroscopy of deuterated pyridine-iron(III) porphyrin complexes. Locations and relaxation times of bound deuterated pyridine resonances.

Deuterium nuclear magnetic resonance spectroscopy of deuterated pyridine-iron(III) porphyrin complexes. Locations and relaxation times of bound deuterated pyridine resonances. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Deuterium nuclear magnetic resonance spectroscopy of deuterated pyridine-iron(III) porphyrin complexes. Locations and relaxation times of bound deuterated pyridine resonances. Abstract Text:

T Shimizu,T Nozawa,M Hatano,

The first application of deuterium nuclear magnetic resonance spectroscopy (2H NMR) to fully deuterated pyridine (d5-pyridine)-iron(III) porphyrin complexes is described. (1) d5-Pyridine gives very broad 2H NMR signals in the presence of hemin or horseradish peroxidase to which pyridine is hardly (or not) bound, probably due to relatively long electronic relaxation times of the high-spin ferric ions. d5-Pyridine in the presence of horse-heart metmyoglobin gives resolved and less broadened 2H NMR signals, probably due to the relatively short electronic relaxation times of the ferric ion and/or to slow chemical exchange of the ligand. (2) The three resonances of free d5-pyridine coalesce into a single or a double resonance in the presence of a heme octapeptide prepared by trypsin digestion of Candida krusei cytochrome c. Nuclear spin-lattice and spin-spin relaxation times of the d5-pyridine-heme octapeptide complex are markedly shorter than those of free d5-pyridine. These findings are interpreted by the chemical exchange mechanism from the temperature dependences of the relaxation times. Thus, on certain assumptions the residence time of pyridine in the bound state and the exchange rate are estimated as approximately 10(-3) s and approximately 400 s-1, respectively, at 25 degrees C. Since 1H NMR signals of axial ligands of paramagnetic hemoproteins are hard to observe, the usefulness of deuterium magnetic resonance for investigating ligand exchange in the paramagnetic hemoproteins is emphasized.

Deuterium nuclear magnetic resonance spectroscopy of deuterated pyridine-iron(III) porphyrin complexes. Locations and relaxation times of bound deuterated pyridine resonances. Publishing Authors By Initials

T Shimizu,T Nozawa,M Hatano,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: pyridines research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: pyridines research

PUBMED ID PMID:

MEDLINE DATE:

Deuterium nuclear magnetic resonance spectroscopy of deuterated pyridine-iron(III) porphyrin complexes. Locations and relaxation times of bound deuterated pyridine resonances. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 91

Page Numbers: 1951-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1982

Deuterium nuclear magnetic resonance spectroscopy of deuterated pyridine-iron(III) porphyrin complexes. Locations and relaxation times of bound deuterated pyridine resonances. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Deuterium nuclear magnetic resonance spectroscopy of deuterated pyridine-iron(III) porphyrin complexes. Locations and relaxation times of bound deuterated pyridine resonances. Keywords Mesh Terms:

KEYWORDS: Pyridines

MESH TERMS:

Chemical & Substance for Abstract: Deuterium nuclear magnetic resonance spectroscopy of deuterated pyridine-iron(III) porphyrin complexes. Locations and relaxation times of bound deuterated pyridine resonances. Information

Substance Name: Deuterium

Registry Number: 7782-39-0

Grant and Affiliation Information for Deuterium nuclear magnetic resonance spectroscopy of deuterated pyridine-iron(III) porphyrin complexes. Locations and relaxation times of bound deuterated pyridine resonances.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Deuterium nuclear magnetic resonance spectroscopy of deuterated pyridine-ironIII porphyrin complexes Locations and relaxation times of bound deuterated pyridine resonances Related Publications

• A fragmin-like protein from plasmodium of Physarum polycephalum that severs F-actin and caps the barbed end of F-actin in a Ca2+-sensitive way.
• Acid denaturation steps of Streptomyces subtilisin inhibitor. A proton magnetic resonance study of individual histidine environment.
• Actin kinase: a protein kinase that phosphorylates actin of fragmin-actin complex.
• Assessment of river water quality during snowmelt and base flow periods in two catchment areas with different land use.
• Axisymmetric analysis of a tube-type acoustic levitator by a finite element method.
• Beta-actinin-like protein from plasmodium.
• Capability of complement fixation of pemphigus antibodies in vitro.
• Chemical modification of subtilisin BPN' by 5-diazo-1-H-tetrazole.
• Deuterium nuclear magnetic resonance spectroscopy of deuterated pyridine-iron(III) porphyrin complexes. Locations and relaxation times of bound deuterated pyridine resonances.
• Dynamic states of the three methionyl residues of Streptomyces subtilisin inhibitor. 1H NMR studies.
• Effect of angiotensin II on noradrenaline content in the rat hypothalamus.
• Electron spin resonance studies on the lipoxygenase reaction by spin trapping and spin labelling methods.
• Episodic release of methane bubbles from peatland during spring thaw.
• Identification of urinary and intestinal bacterial metabolites of ellagitannin geraniin in rats.
• Inactivation of Rhodotorula glutinis acid protease by diazoacetyl compounds.
• Inactivation of subtilism BPN' by N-bromosuccinimide.
• Isolation and characterization of Physarum profilin.
• Mechanism of the ropivacaine-induced increase in intracellular Ca2+ concentration in rat aortic smooth muscle.
• Nitric oxide: involvement in the effects of anesthetic agents.
• Propofol restores brain microvascular function impaired by high glucose via the decrease in oxidative stress.
• Stoichiometric Mg:LiNbO(3) as an effective material for nonlinear optics.
• Subsite affinities of bacterial liquefying alpha-amylase evaluated from the rate parameters of linear substrates.
• The mechanism of intestinal motility in homozygous mutant Ncx/Hox11L.1-deficient mice--a model for intestinal neuronal dysplasia.
• [Four cases of mitral valve prolapse associated with ventricular tachycardia]
• [Human science. Sex. 6. Sexuality]
• [Long-term vasodilator therapy of chronic refractory heart failure in patients with dilated cardiomyopathy]
• [Psychology of the wife who is facing her husband's death and the role of nursing]
• [Significance of the nursing record and its key points - a thought in the nursing process]
• [Teaching of the "nursing process": the "nursing process" in relation to the theory and practice]
• [The family. 12. The problems of family members around the patient and their interference with nursing]