Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation.

Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation. Research Abstract Details 

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Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation. Abstract Text:

John Hanna,Nathaniel A Hathaway,Yoshiko Tone,Bernat Crosas,Suzanne Elsasser,Donald S Kirkpatrick,David S Leggett,Steven P Gygi,Randall W King,Daniel Finley,

Ubiquitin chains serve as a recognition motif for the proteasome, a multisubunit protease, which degrades its substrates into polypeptides while releasing ubiquitin for reuse. Yeast proteasomes contain two deubiquitinating enzymes, Ubp6 and Rpn11. Rpn11 promotes protein breakdown through its degradation-coupled activity. In contrast, we show here that Ubp6 has the capacity to delay the degradation of ubiquitinated proteins by the proteasome. However, delay of degradation by Ubp6 does not require its catalytic activity, indicating that Ubp6 has both deubiquitinating activity and proteasome-inhibitory activity. Delay of degradation by Ubp6 appears to provide a time window allowing gradual deubiquitination of the substrate by Ubp6. Rpn11 catalyzes en bloc chain removal, and Ubp6 interferes with degradation at or upstream of this step, so that degradation delay by Ubp6 is accompanied by a switch in the mode of ubiquitin chain processing. We propose that Ubp6 regulates both the nature and magnitude of proteasome activity.

Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation. Publishing Authors By Initials

J Hanna,NA Hathaway,Y Tone,B Crosas,S Elsasser,DS Kirkpatrick,DS Leggett,SP Gygi,RW King,D Finley,

For similar proteins: ubiquitins: ubiquitin research abstracts see: proteins: ubiquitins: ubiquitin research

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Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cell

VOLUME: 127

Page Numbers: 99-111

Journal Abbreviation: Cell

ISSN: 0092-8674

DAY: 6

MONTH: Oct

YEAR: 2006

Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 413066

Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation. Keywords Mesh Terms:

KEYWORDS: Ubiquitin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation. Information

Substance Name: UBP6 protein, S cerevisiae

Registry Number: EC 3.4.99.-

Grant and Affiliation Information for Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation.

AFFILIATION: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM67945

ACRONYM: GM

MEDLINETA: Cell

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