Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy.

Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy. Research Abstract Details 

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Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy. Abstract Text:

Yan Chen,Joachim D ,

The brightness of fluorescence fluctuations provides information about protein interactions in the intercellular environment under equilibrium conditions. Here we demonstrate that the stoichiometry of a protein complex containing two proteins labeled with CFP and YFP can be determined by brightness analysis. The brightness profile, which characterizes the brightness as a function of the labeled protein coexpression ratio, together with brightness titration experiments, provides sufficient information to quantify the composition of a protein complex under stoichiometric binding conditions. The selective and simultaneous excitation of proteins labeled with CFP and YFP by choosing different excitation wavelengths is used to identify the composition of the protein complex. Interactions between nuclear receptors and their coactivators play a crucial role in the regulation of gene expression. We choose the ligand-binding domain of retinoic X receptor and the nuclear receptor interacting domain of the steroid receptor coactivator-1 as a model for exploring the formation of a hetero-oligomer by brightness analysis directly in living cells. Our results show the formation of a heterotetramer with three nuclear receptors binding to the coactivator domain. Elimination of one of the nuclear receptor binding sites through a truncation mutant changed the interaction between both proteins significantly and led to a nuclear receptor-induced oligomerization of the truncated coactivator. Quantifying protein interactions in a cell is an important step in understanding cellular function on a molecular level. This study provides proof-of-principle experiments that illustrate the potential of brightness analysis as a powerful tool for quantifying protein interactions in living cells.

Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy. Publishing Authors By Initials

Y Chen,JD ,

For similar investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research abstracts see: investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research

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Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 3147-52

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 16

MONTH: 02

YEAR: 2007

Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Fluorescence

MESH TERMS: methods

Chemical & Substance for Abstract: Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy. Information

Substance Name: Retinoid X Receptors

Registry Number: 0

Grant and Affiliation Information for Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy.

AFFILIATION: School of Physics and Astronomy, University of Minnesota, 116 Church Street Southeast, Minneapolis, MN 55455, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM64589

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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