Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism.

Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism. Research Abstract Details 

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Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism. Abstract Text:

Norma J Greenfield,Norma J Greenfield,

Circular dichroism (CD) is an excellent tool for examining the interactions and stability of proteins. This protocol covers methods to obtain and analyze circular dichroism spectra to measure changes in the folding of proteins as a function of denaturants, osmolytes or ligands. Applications include determination of the free energy of folding of a protein, the effects of mutations on protein stability and the estimation of binding constants for the interactions of proteins with other proteins, DNA or ligands, such as substrates or inhibitors. The experiments require 2-5 h.

Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism. Publishing Authors By Initials

NJ Greenfield,NJ Greenfield,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein folding research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein folding research

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Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature protocols

VOLUME: 1

Page Numbers: 2733-41

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2006

Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism. Information

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LANGUAGE: eng

NlmUniqueID: 101284307

Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism. Keywords Mesh Terms:

KEYWORDS: Protein Folding

MESH TERMS: methods

Chemical & Substance for Abstract: Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism. Information

Substance Name: Ligands

Registry Number: 0

Grant and Affiliation Information for Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism.

AFFILIATION: Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, New Jersey 08854-8021, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM-36326

ACRONYM: GM

MEDLINETA: Nat Protoc

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